AGO2

Gene Symbol AGO2
Entrez Gene 27161
Alt Symbol EIF2C2, Q10
Species Human
Gene Type protein-coding
Description argonaute RISC catalytic component 2
Other Description CTA-204B4.6|PAZ Piwi domain protein|PPD|argonaute 2|argonaute2|eIF-2C 2|eIF2C 2|eukaryotic translation initiation factor 2C, 2|hAgo2|protein argonaute-2|protein slicer
Swissprots Q8WV58 Q96ID1 Q8TCZ5 Q9UKV8
Accessions CAK32193 EAW92207 EAW92208 Q9UKV8 AA909234 AF121255 AAF13034 AI468419 AK096164 AK130012 AL050151 AY077717 AAL76093 BC007633 AAH07633 BC018727 AAH18727 BC054491 BC065217 BC125213 AAI25214 BC125214 BC160104 AAI60104 BT007229 AAP35893 HG501752 XM_011516964 XP_011515266 XM_011516965 XP_011515267 XM_011516966 XP_011515268 XM_011516967 XP_011515269 XM_011516968 XP_011515270 NM_001164623 NP_001158095 NM_012154 NP_036286
Function Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to t
Subcellular Location Cytoplasm, P-body. Nucleus. Note=Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8.

Argonaute 2 (C34C6) Rabbit mAb - 2897 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C34C6
Western blot analysis of extracts from various cell types using Argonaute 2 (C34C6) Rabbit mAb.

eIF2C (B-3) - sc-376696 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
eIF2C (B-3): sc-376696. Western blot analysis of eIF2C expression in K-562 (A) and HeLa (B) whole cell lysates.

eIF2C (H-300) - sc-32877 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of eIF2C expression in K-562 (A) and HeLa (B) whole cell lysates immunoprecipitated with eIF2C (4F9): sc-53521 and detected with eIF2C (H-300): sc-32877.

eIF2C (N-16) - sc-32360 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
eIF2C (N-16): sc-32360. Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells.

eIF2C2 (4F9) - sc-53521 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of eIF2C expression in K-562 (A) and HeLa (B) whole cell lysates immunoprecipitated with eIF2C (4F9): sc-53521 and detected with eIF2C (H-300): sc-32877.

eIF2C2 (N-13) - sc-32659 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
eIF2C2 (N-13): sc-32659. Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells.

Anti-Ago2 / eIF2C2 antibody - ab135025 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-Ago2 / eIF2C2 antibody (ab135025) at 1/1000 dilutionLane 1 : HeLa cell extractLane 2 : mouse MEF cell extract

Anti-Ago2 / eIF2C2 antibody [EPR10410] - ab156870 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10410
All lanes : Anti-Ago2 / eIF2C2 antibody [EPR10410] (ab156870) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF7 cell lysateLane 3 : HepG2 cell lysateLane 4 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-Ago2 / eIF2C2 antibody [EPR10410] (Alexa Fluor® 488) - ab196469 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10410
Overlay histogram showing HeLa cells stained with ab196469 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196469, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Ago2 / eIF2C2 antibody [EPR10410] (Alexa Fluor® 647) - ab196516 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10410
ab196516 staining Ago2 / eIF2C2 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196516 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Ago2 / eIF2C2 antibody [EPR10410] (HRP) - ab195892 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10410
All lanes : Anti-Ago2 / eIF2C2 antibody [EPR10410] (HRP) (ab195892) at 1/5000 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-Ago2 / eIF2C2 antibody [EPR10411] - ab186733 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10411
All lanes : Anti-Ago2 / eIF2C2 antibody [EPR10411] (ab186733) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF7 cell lysateLane 3 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Anti-Ago2 / eIF2C2 antibody - ChIP Grade - ab32381 from Abcam

Prices $401.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Ago2 / eIF2C2 antibody - ChIP Grade (ab32381) at 2 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLysates/proteins at 20 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Ago2 / eIF2C2 antibody - ChIP Grade - ab57113 from Abcam

Prices $401.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Ago2 / eIF2C2 antibody (ab57113) used in immunofluorescence at 10ug/ml on HeLa cells.

Anti-AGO2 / EIF2C2 Antibody (clone 2E12-1C9) IHC-plus™ - LS-B6224 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2E12-1C9
Anti-EIF2C2 / AGO2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6224 concentration 3 ug/ml.

Anti-AGO2 / EIF2C2 Antibody - LS-C69256 from LifeSpan Bioscience

Prices $530.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-AGO2 / EIF2C2 Antibody - LS-C128715 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal

Anti-AGO2 / EIF2C2 Antibody (N-Terminus, APC) - LS-C222370 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AGO2 / EIF2C2 Antibody (N-Terminus, Biotin) - LS-C222372 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AGO2 / EIF2C2 Antibody (N-Terminus, AP) - LS-C222371 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AGO2 / EIF2C2 Antibody (N-Terminus, FITC) - LS-C222373 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AGO2 / EIF2C2 Antibody (N-Terminus, Azide-free, HRP) - LS-C222374 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AGO2 / EIF2C2 Antibody (N-Terminus, PE) - LS-C222375 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AGO2 / EIF2C2 Antibody (aa498-812) - LS-C311886 from LifeSpan Bioscience

Prices $250.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-AGO2 / EIF2C2 Antibody (N-Terminus) - LS-C329490 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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