All lanes : Anti-EHD2 Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysates Lane 2: A549 whole cell lysates Lane 3: human placenta lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis of paraffin-embedded H. placenta section using EHD2 Antibody (C-term)(Cat# AW5422). AW5422 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. heart section using EHD2 Antibody (C-term)(Cat# AW5422). AW5422 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.