Anti-AHCYL1 antibody
| Name | Anti-AHCYL1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56761 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Applications | WB IHC-P FC |
| Species Reactivities | Chicken, Human |
| Antigen | Recombinant fragment, corresponding to amino acids 1-102 of Human AHCYL1 |
| Description | Mouse Monoclonal |
| Gene | AHCYL1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab56761 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56761, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/3727_ab56761-238329-ab56761FC.jpg)
Overlay histogram showing HeLa cells stained with ab56761 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56761, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
AHCYL1 antibody (ab56761) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.
Product References
Paradoxical expression of AHCYL1 affecting ovarian carcinogenesis between - Paradoxical expression of AHCYL1 affecting ovarian carcinogenesis between
Jeong W, Kim HS, Kim YB, Kim MA, Lim W, Kim J, Jang HJ, Suh DH, Kim K, Chung HH, Bazer FW, Song YS, Han JY, Song G. Exp Biol Med (Maywood). 2012 Jul;237(7):758-67.
AHCYL1 is mediated by estrogen-induced ERK1/2 MAPK cell signaling and microRNA - AHCYL1 is mediated by estrogen-induced ERK1/2 MAPK cell signaling and microRNA
Jeong W, Kim J, Ahn SE, Lee SI, Bazer FW, Han JY, Song G. PLoS One. 2012;7(11):e49204.