AHCYL1

Gene Symbol AHCYL1
Entrez Gene 10768
Alt Symbol DCAL, IRBIT, PPP1R78, PRO0233, XPVKONA
Species Human
Gene Type protein-coding
Description adenosylhomocysteinase-like 1
Other Description DC-expressed AHCY-like molecule|IP(3)Rs binding protein released with IP(3)|S-adenosyl homocysteine hydrolase homolog|S-adenosyl-L-homocysteine hydrolase 2|S-adenosylhomocysteine hydrolase-like protein 1|adoHcyase 2|dendritic cell expressed AHCY-like protein|inositol 1,4,5-trisphosphate receptor-binding protein|protein phosphatase 1, regulatory subunit 78|putative adenosylhomocysteinase 2
Swissprots Q2TAJ6 Q5VSM0 Q502W8 B4E168 Q9UG84 Q6P171 Q96PK4 O43865
Accessions CAH70965 CAH70966 CAV30704 EAW56422 EAW56423 EAW56424 EAW56425 EAW56426 EAW56427 O43865 AA427939 AA865152 AF035319 AAB88189 AF090905 AAF24028 AF315687 AAL26869 AK131563 BAD18696 AK303690 BAG64680 AK308080 AK313392 BAG36190 AK316110 BAH14481 AL049954 CAB43223 BC003631 AAH03631 BC007576 AAH07576 BC010681 AAH10681 BC016942 AAH16942 BC065254 AAH65254 BC095476 AAH95476 BC110896 AAI10897 BC111563 AAI11564 BC140414 AAI40415 BC146492 AAI46493 BK005417 DAA05762 BK005418 DAA05763 BQ774807 CF456195 DC328766 DC329490 DQ891021 ABM81947 DQ894201 ABM85127 EU831760 ACE87278 EU831838 ACE86597 U82761 AAC01960 XM_011540535 XP_011538837 XR_946527 NM_001242673 NP_001229602 NM_001242674 NP_001229603 NM_001242675 NP_001229604 NM_001242676 NP_001229605 NM_006621 NP_006612
Function Acts as a (dATP)-dependent inhibitor of ribonucleotide reductase large subunit RRM1, controlling the endogenous dNTP pool and ensuring normal cell cycle progression. {ECO:0000269|PubMed:25237103}.
Subcellular Location Endoplasmic reticulum {ECO:0000250}.
Tissue Specificity Expressed in dendritic cells. {ECO:0000269|PubMed:11904675}.
Top Pathways Cysteine and methionine metabolism

AHCYL1/2 (D-7) - sc-271581 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
AHCYL1/2 (D-7): sc-271581. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

AHCYL1/2 (H-160) - sc-134650 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
AHCYL1/2 (H-160): sc-134650. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

AHCYL1 (N-15) - sc-162507 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AHCYL1 (N-15): sc-162507. Western blot analysis of AHCYL1 expression in non-transfected: sc-117752 (A) and mouse AHCYL1 transfected: sc-118278 (B) 293T whole cell lysates.

Anti-AHCYL1 antibody - ab56761 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HeLa cells stained with ab56761 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56761, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-AHCYL1 antibody - ab77638 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-AHCYL1 antibody (ab77638) at 1/500 dilution + Recombinant fragment of human AHCYL1 at 0.2 µgSecondaryGoat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

Anti-AHCYL1 antibody - ab172513 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AHCYL1 antibody (ab172513) at 1 µg/mlLane 1 : AHCYL1 transfected 293T lysateLane 2 : Non-transfected 293T lysateLysates/proteins at 15 µl per lane.SecondaryHRP Goat anti Rabbit at 1/2500 dilutiondeveloped using the ECL technique

Anti-AHCYL1 antibody - ab82671 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-AHCYL1 antibody (ab82671) at 1 µg/ml + human fetal brain lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-AHCYL1 antibody [EPR13409] - ab178693 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13409
All lanes : Anti-AHCYL1 antibody [EPR13409] (ab178693) at 1/10000 dilutionLane 1 : Human thymus lysateLane 2 : HeLa lysateLane 3 : Ramos lysateLane 4 : 293T lysateLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/2000 dilution

Anti-AHCYL1 / DCAL Antibody (clone 5D6) - LS-C133660 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5D6
Immunoperoxidase of monoclonal antibody to AHCYL1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]

Anti-AHCYL1 / DCAL Antibody - LS-C309360 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-AHCYL1 / DCAL Antibody (aa72-121) - LS-C80045 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-AHCYL1 / DCAL Antibody (N-Terminus) - LS-C80046 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-AHCYL1 / DCAL Antibody (C-Terminus) - LS-C137124 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

AHCYL1 Antibody (5D6) - H00010768-M05 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5D6
Western Blot: AHCYL1 Antibody (5D6) [H00010768-M05] - Analysis of AHCYL1 expression in HeLa (Cat # L013V1).

AHCYL1 Antibody - NBP1-83093 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: AHCYL1 Antibody [NBP1-83093] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

AHCYL1 Antibody - NBP1-57655 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal

AHCYL1 Antibody - NBP1-57654 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal

AHCYL1 Antibody (1B3) - H00010768-M03 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1B3
Western Blot: AHCYL1 Antibody (1B3) [H00010768-M03] - Analysis of AHCYL1 expression in HeLa (Cat # L013V1).

AHCYL1 Antibody - NBP2-15308 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: AHCYL1 Antibody [NBP2-15308] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE; antibody diluted at 1:1000.

AHCYL1 Antibody - H00010768-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

AHCYL1 Antibody - H00010768-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

Anti-AHCYL1 antibody produced in rabbit - SAB1410126 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-AHCYL1: <B>Cat. No. SAB1410126</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~59 kDa using AHCYL1 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-AHCYL1 (ab2) antibody produced in rabbit - SAB2100077 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-AHCYL1 (ab2): <B>Cat. No.SAB2100077</B>: AHCYL1 in Placenta tissue lysate with AHCYL1 antibody at 1.0 μg/mL.

Anti-AHCYL1 (ab1) antibody produced in rabbit - SAB2100076 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-AHCYL1 (ab1): <B>Cat. No.SAB2100076</B>: AHCYL1 in Fetal Brain tissue lysate with AHCYL1 antibody at 1.0 μg/mL.

Anti-AHCYL1 antibody produced in rabbit - HPA042589 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-AHCYL1: <B>Cat. No. HPA042589</B>: Immunohistochemical staining of human pancreas shows moderate cytoplasmic and membranous positivity in ducts.
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