All lanes : Anti-AK2 antibody [EPR11388(B)] (ab166901) at 1/1000 dilutionLane 1 : Fetal kidney lysateLane 2 : Fetal liver lysateLane 3 : HepG2 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP antibody at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling AK2 using ab166901 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling AK2 using ab166901 at 1/100 dilution.
Immunofluorescent analysis of HepG2 cells labeling AK2 using ab166901 at 1/100 dilution.
Immunoprecipitation of AK2 from HeLa cell lysate pellet using ab166901 at 1/10 dilution followed by immunoblotting. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.