All lanes : Anti-AKAP1 antibody (ab69580) at 1/500 dilutionLane 1 : extracts from COLO cells without immunising peptideLane 2 : extracts from HUVEC cells without immunising peptideLane 3 : extracts from COLO cells with immunising peptideLysates/proteins at 30 µg per lane.Observed band size : 92 kDa (why is the actual band size different from the predicted?)
Immunohistochemical analysis of AKAP1 in paraffin embedded human brain tissue using 1/50 ab69580. Left image: untreated sample. Right image: sample treated with immunising peptide.
Immunofluorescence analysis of AKAP1 in COS7 cells, using 1/500 ab69580. Left image: untreated cells. Right image: cells treated with immunising peptide.