AKAP1

Gene Symbol	AKAP1
Entrez Gene	8165
Alt Symbol	AKAP, AKAP121, AKAP149, AKAP84, D-AKAP1, PPP1R43, PRKA1, SAKAP84, TDRD17
Species	Human
Gene Type	protein-coding
Description	A kinase (PRKA) anchor protein 1
Other Description	A-kinase anchor protein 1, mitochondrial\|A-kinase anchor protein 149 kDa\|AKAP 149\|D-AKAP-1\|dual-specificity A-kinase anchoring protein 1\|protein kinase A anchoring protein 1\|protein kinase A1\|protein phosphatase 1, regulatory subunit 43\|spermatid A-kinase anchor protein 84\|tudor domain containing 17
Swissprots	Q13320 D3DTZ0 Q9BW14 Q92667 A8K8Q1
Accessions	EAW94515 EAW94516 EAW94517 EAW94518 Q92667 AF015910 AAB64298 AK292416 BAF85105 AK297810 BAG60148 AK308397 BC000729 AAH00729 BM711930 CB048239 CD172211 DA092401 DA455114 DQ893602 ABM84528 EU176646 ABW03447 U34074 AAC50279 X97335 CAA66000 XM_005257707 XP_005257764 XM_005257709 XP_005257766 XM_011525318 XP_011523620 NM_001242902 NP_001229831 NM_001242903 NP_001229832 NM_003488 NP_003479 NM_139275
Function	Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane.
Subcellular Location	Mitochondrion outer membrane {ECO:0000305}.
Tissue Specificity	AKAP149 is highly expressed in prostate and small intestine whereas S-AKAP84 is expressed in kidney, pancreas, liver, lung and brain. AKAP149 is also expressed in colon carcinoma.

AKAP1 (D9C5) XP ® Rabbit mAb - 5203 from Cell Signaling Technology

Prices	$109.00, $277.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D9C5

Western blot analysis of extracts from various cell lines using AKAP1 (D9C5) XP ® Rabbit mAb.

AKAP 149 (H-8) - sc-390072 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

AKAP 149 (H-8): sc-390072. Western blot analysis of AKAP 149 expression in LNCaP whole cell lysate.

AKAP 149/121 (B-10) - sc-377450 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

AKAP 149/121 (B-10): sc-377450. Western blot analysis of AKAP 149/121 expression in HeLa (A) and LNCaP (B) whole cell lysates.

AKAP 149/121 (C-20) - sc-6439 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

AKAP 149/121 (C-20): sc-6439. Western blot analysis of AKAP 149/121 expression in Hep G2 whole cell lysate.

AKAP 149/121 (H-300) - sc-33576 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

AKAP 149 (H-300): sc-33576. Western blot analysis of AKAP 149 expression in non-transfected: sc-117752 (A) and mouse AKAP 149 transfected: sc-178271 (B) 293T whole cell lysates.

AKAP 149/121 (22) - sc-136355 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

AKAP 149/121 (6) - sc-135824 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	50 µg/0.5 ml
Host	Mouse
Clonality	Monoclonal

AKAP 149/121 (6): sc-135824. Western blot analysis of AKAP 149/121 expression in HeLa whole cell lysate.

Anti-AKAP1 antibody - ab69580 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-AKAP1 antibody (ab69580) at 1/500 dilutionLane 1 : extracts from COLO cells without immunising peptideLane 2 : extracts from HUVEC cells without immunising peptideLane 3 : extracts from COLO cells with immunising peptideLysates/proteins at 30 µg per lane.Observed band size : 92 kDa (why is the actual band size different from the predicted?)

Anti-AKAP1 antibody - ab85588 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-AKAP1 antibody (ab85588) at 1 µg/ml + fetal brain lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-AKAP1 antibody [EPR10518] - ab156004 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR10518

All lanes : Anti-AKAP1 antibody [EPR10518] (ab156004) at 1/1000 dilutionLane 1 : SW480 cell lysateLane 2 : Caco-2 cell lysateLane 3 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-AKAP1 / AKAP Antibody - LS-C306168 from LifeSpan Bioscience

Prices	$455.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Anti-AKAP1 / AKAP Antibody (aa450-500) - LS-C288174 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human AKAP1 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-AKAP1 used at a dilution of 1:200 (1 ug/ml).

Anti-AKAP1 / AKAP Antibody (aa541-571) - LS-C101624 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

AKAP1 antibody LS-C101624 Western blot of Fetal Brain lysate.

Anti-AKAP1 / AKAP Antibody (aa281-330) - LS-C119182 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Immunofluorescence of COS7 cells, using AKAP1 Antibody. The picture on the right is treated with the synthesized peptide.

Anti-AKAP1 / AKAP Antibody - LS-C334405 from LifeSpan Bioscience

Prices	$305.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts of various cell lines, using AKAP1 antibody.

Anti-AKAP1 / AKAP Antibody (aa375-425) - LS-C288171 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human AKAP1 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-AKAP1 used at a dilution of 1:100 (2 ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody.

Anti-AKAP1 / AKAP Antibody (Asn306) - LS-C177027 from LifeSpan Bioscience

Prices	$345.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

IHC of AKAP 149(N306) pAb in paraffin-embedded human brain tissue.

Anti-AKAP1 / AKAP Antibody (aa375-425) - LS-C285945 from LifeSpan Bioscience

Prices	$415.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

AKAP1 Antibody - NBP1-89172 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: AKAP1 Antibody [NBP1-89172] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

AKAP1 Antibody - NBP2-15319 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: AKAP1 Antibody [NBP2-15319] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE; antibody diluted at 1:10000.

AKAP1 Antibody - NBP1-19690 from Novus Biologicals

Prices	$349.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry-Paraffin: AKAP1 Antibody [NBP1-19690] - Paraffin-embedded human brain tissue.

AKAP1 Antibody - 27850002 from Novus Biologicals

Prices	$369.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry: AKAP1 Antibody [27850002] - Most normal tissues and malignant tissues showed strong cytoplasmic immunoreactivity. Decidual cells in placenta were moderately stained. Bile ducts, renal glomeruli and stromal cells along with some of the malignant lymphomas and renal cancers were weakly stained or negative. Image and statement courtesy of the Human Protein Atlas (HPA).

AKAP1 Antibody - NBP1-33629 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: AKAP1 Antibody [NBP1-33629] - Western blot analysis of AKAP1 on 293T lysates using NBP1-33629 at a dilution of 1:1000. 30ug whole cell lysate loaded on 7.5% SDS PAGE gel.

AKAP1 Antibody - H00008165-A01 from Novus Biologicals

Prices	$269.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Western Blot: AKAP1 Antibody [H00008165-A01] - Western Blot analysis of AKAP1 expression in HeLa ( Cat # L013V1 ).

AKAP1 Antibody - H00008165-B01P from Novus Biologicals

Prices	$359.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Western Blot: AKAP1 Antibody [H00008165-B01P] - Analysis of AKAP1 expression in transfected 293T cell line by AKAP1 polyclonal antibody. Lane 1: AKAP1 transfected lysate(99.33 KDa). Lane 2: Non-transfected lysate.

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