All lanes : Anti-AKR1B10 antibody (ab96417) at 1/1000 dilutionLane 1 : MOLT4 whole cell lysateLane 2 : Raji whole cell lysateLysates/proteins at 30 µg per lane.
ab96417, at 1/200 dilution, staining AKR1B10 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. The lower image is merged with DNA probe.
ab96417, at 1/500 dilution, staining AKR1B10 in paraffin-embedded OVCAR3 xenograft sections by Immunohistochemistry.
ab96417 staining AKR1B10 in Human endometrial cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 60 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 20 hours at 4°C. A Goat anti-rabbit IgG + IgM H+L polyclonal (1/100) was used as the secondary antibody.See Abreview