Anti-AKT1 (phospho S473) antibody
| Name | Anti-AKT1 (phospho S473) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab18206 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB |
| Species Reactivities | Mouse, Monkey, Rat, Bovine, Human, Xenopus |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human AKT, phosphorylated at S473 |
| Blocking Peptide | Human pan-AKT (phospho S473) peptide |
| Description | Rabbit Polyclonal |
| Gene | AKT1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of AKT1 (phospho S473) staining in mouse lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18206, 5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-AKT1 (phospho S473) antibody (ab18206) at 1 µg/mlLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 2 : NIH 3T3 treated with Vanadate and PDGF Whole Cell LysateLane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Human pan-AKT (phospho S473) peptide (ab18622) at 1 µg/mlLane 4 : NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate with Human pan-AKT (phospho S473) peptide (ab18622) at 1 µg/mlLane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Human AKT1 (unmodified ) peptide (ab24649) at 1 µg/mlLane 6 : NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate with Human AKT1 (unmodified ) peptide (ab24649) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
Product References
Folic acid modulates eNOS activity via effects on posttranslational modifications - Folic acid modulates eNOS activity via effects on posttranslational modifications
Taylor SY, Dixon HM, Yoganayagam S, Price N, Lang D. Eur J Pharmacol. 2013 Aug 15;714(1-3):193-201.
Negative regulation of Wnt signaling mediated by CK1-phosphorylated Dishevelled - Negative regulation of Wnt signaling mediated by CK1-phosphorylated Dishevelled
Witte F, Bernatik O, Kirchner K, Masek J, Mahl A, Krejci P, Mundlos S, Schambony A, Bryja V, Stricker S. FASEB J. 2010 Jul;24(7):2417-26.