AKT1

Gene Symbol AKT1
Entrez Gene 280991
Alt Symbol -
Species Bovine
Gene Type protein-coding
Description v-akt murine thymoma viral oncogene homolog 1
Other Description PKB alpha|RAC-PK-alpha|RAC-alpha serine/threonine-protein kinase|protein kinase B alpha|v-akt murine thymoma viral oncogene-like protein 1
Swissprots Q5ER96 Q01314
Accessions DAA17274 Q01314 AY781100 AAW71957 X61036 CAA43371 NM_173986 NP_776411
Function AKT1 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glyco
Subcellular Location Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. Cell membrane {ECO:0000250}. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A (By similarity). Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus (By similarity). {ECO:0000250}.
Top Pathways Apoptosis, Tight junction, VEGF signaling pathway, Focal adhesion, Toll-like receptor signaling pathway

Anti-AKT1 (phospho S473) antibody - ab8932 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Normal breast tissue. Akt is weakly phosphorylated in normal tissue in the breast. The phosphorylated Akt is clearly localised in the cytoplasm.The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.

Anti-AKT1 (phospho S473) antibody - ab18206 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of AKT1 (phospho S473) staining in mouse lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18206, 5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-AKT1 antibody [4D6] - ab124341 from Abcam

Prices $394.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4D6
All lanes : Anti-AKT1 antibody [4D6] (ab124341) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY AKT1 cDNALysates/proteins at 5 µg per lane.

Anti-AKT1 antibody [E45] - ab32038 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E45
Immunohistochemistry (Paraffin-embedded sections) analysis using ab32038 at a dilution of 1/1000 - 1/1250 and squamous lung carcinoma

Anti-AKT1 antibody [Y89] - ab32505 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y89
ab32505 staining AKT1 in SK-N-SH cells treated with alsterpaullone (ab141070), by ICC/IF. Decrease of AKT1 expression correlates with increased concentration of alsterpaullone, as described in literature.The cells were incubated at 37°C for 6h in media containing different concentrations of ab141070 (alsterpaullone) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32505 (1/200 dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Anti-AKT1 antibody [Y90] - ab32510 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y90
Anti-AKT1 antibody [Y90] (ab32510) at 1/1000 dilution + MCF7 cell lysate

V-Akt Murine Thymoma Viral Oncogene Homolog 1 Antibody - abx116581 from Abbexa

Host Rabbit
Clonality Polyclonal

AKT1 Antibody - CSB-PA001553GA01HU from Cusabio

Host Rabbit
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