All lanes : Anti-AKT1/2/3 (phospho Y315 + Y316 + Y312) antibody (ab131443) at 1/500 dilutionLane 1 : HepG2 cell extract untreatedLane 2 : HepG2 cell extract treated with EGF
Immunofluorescent analysis of methanol-fixed HeLa cells labelling AKT1/2/3 (phospho Y315 + Y316 + Y312) with ab131443 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling AKT1/2/3 (phospho Y315 + Y316 + Y312) with ab131443 at 1/50 dilution. Right panel was preincubated with blocking peptide.