AKT3

Gene Symbol AKT3
Entrez Gene 10000
Alt Symbol MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2
Species Human
Gene Type protein-coding
Description v-akt murine thymoma viral oncogene homolog 3
Other Description PKB gamma|RAC-gamma serine/threonine protein kinase|RAC-gamma serine/threonine-protein kinase|v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
Swissprots Q96QV3 Q5VTI1 Q5VTI2 Q9UFP5 Q9Y243 Q0VAA6
Accessions CAC22382 CAC93797 EAW77093 EAW77094 Q9Y243 AF085234 AAL40392 AF124141 AAD29089 AF135794 AAD24196 AJ245709 CAB53537 AK055109 AK308052 AL117525 CAB55977 AM392791 CAL37669 AM392849 CAL37727 AW960221 AY005799 AAF91073 BC019085 BC020479 AAH20479 BC121154 AAI21155 BG702262 U79271 XM_005272994 XP_005273051 XM_005272995 XP_005273052 XM_005272997 XP_005273054 XM_006711726 XP_006711789 XM_006724959 XP_006725022 XM_006724960 XP_006725023 XM_006724962 XP_006725025 XM_006724963 XP_006725026 XM_011544011 XP_011542313 XM_011544012 XP_011542314 XM_011544013 XP_011542315 XM_011544014 XP_011542316 XM_011546424 XP_011544726 XM_011546425 XP_011544727 XM_011546426 XP_011544728 XM_011546427 XP_011544729 NM_001206729 NP_001193658 NM_005465 NP_005456 NM_181690 NP_859029
Function AKT3 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase- dependent apoptosis. {ECO:0000269|PubMed:18524868, EC
Subcellular Location Nucleus {ECO:0000269|PubMed:20018949}. Cytoplasm {ECO:0000269|PubMed:20018949}. Membrane {ECO:0000269|PubMed:20018949}; Peripheral membrane protein {ECO:0000269|PubMed:20018949}. Note=Membrane-associated after cell stimulation leading to its translocation.
Tissue Specificity In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
Top Pathways Apoptosis, Tight junction, VEGF signaling pathway, Focal adhesion, Toll-like receptor signaling pathway

Akt3 (L47B1) Mouse mAb - 8018 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone L47B1
Western blot analysis of extracts from various cell lines using Akt3 (L47B1) Mouse mAb (upper) and Akt (pan) (40D4) Mouse mAb #2920 (lower).

Akt3 (E2B6R) Rabbit mAb - 14293 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone E2B6R
Western blot analysis of extracts from A172 and MCF7 cells using Akt3 (E2B6R) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

Akt3 Antibody - 4059 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from CAD and PC12 cells, using Akt3 Antibody.

Akt3 (62A8) Rabbit mAb - 3788 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 62A8
Western blot analysis of recombinant Akt1, Akt2 and Akt3 proteins, and extracts from MDA-MB-231 (human), CAD (mouse) and PC12 (rat) cells, using Akt3 (62A8) Rabbit mAb (upper) or Akt Antibody #9272 (lower). Recombinant Akt1, Akt2 and Akt3 are 6His-fusion proteins, MW=66 kDa.

Akt3 (C-14) - sc-11520 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Akt3 (C-14): sc-11520. Western blot analysis of Akt3 expression in insulin treated NIH/3T3 whole cell lysate.

Akt3 (M-14) - sc-11521 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Akt3 (M-14): sc-11521. Western blot analysis of Akt3 expression in mouse brain tissue extract.

Akt3 (EE-M14) - sc-134254 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Akt3 (EE-M14): sc-134254. Western blot analysis of Akt3 expression in human Akt3 transfected (A) and non-transfected (B) 293T whole cell lysates.

Anti-AKT1/2/3 (phospho S472 + S473 + S474) antibody - ab183758 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AKT1/2/3 (phospho S472 + S473 + S474) antibody (ab183758) at 1/500 dilutionLane 1 : MCF7 whole cell lysate/extract (untreated)Lane 2 : MCF7 whole cell lysate/extract (500nM PMA treatment for 1hr)Lane 3 : MCF7 whole cell lysate/extract (500nM PMA treatment for 2hrs)Lane 4 : MCF7 whole cell lysate/extract (500nM PMA treatment for 4hrs)Lane 5 : MCF7 whole cell lysate/extract (500nM PMA treatment for 6hrs)Lysates/proteins at 30 µg per lane.

Anti-AKT1/2/3 (phospho Y315 + Y316 + Y312) antibody - ab131443 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AKT1/2/3 (phospho Y315 + Y316 + Y312) antibody (ab131443) at 1/500 dilutionLane 1 : HepG2 cell extract untreatedLane 2 : HepG2 cell extract treated with EGF

Anti-AKT1/2/3 (phospho Y315 + Y316 + Y312) antibody - ab111414 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using AKT1/2/3 (Phospho-Tyr315/316/312) antibody (ab111414). The picture on the right is treated with the synthesized phospho peptide.

Anti-AKT1/2/3 antibody - ab106693 from Abcam

Prices $407.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab106693, at 1/100 dilution, staing AKT1/2/3 in Human liver followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-AKT1/2/3 antibody - ab79360 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AKT1/2/3 antibody (ab79360) at 1/500 dilutionLane 1 : A549 cell extract at 5 µgLane 2 : A549 cell extract with immunizing peptide at 5 µg

Anti-AKT1/2/3 antibody - ab126811 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AKT1/2/3 antibody (ab126811) at 1/5000 dilutionLane 1 : Rat vascular smooth muscle cell lysateLane 2 : Rat vascular smooth muscle cell lysateLysates/proteins at 100000 cells per lane.SecondaryHRP-conjugated donkey anti-rabbit IgG polyclonal at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-AKT1/2/3 antibody - ab82538 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AKT1/2/3 antibody (ab82538) at 1/1000 dilutionLane 1 : human recombinant AKT1Lane 2 : human recombinant AKT2Lane 3 : human recombinant AKT3Lysates/proteins at 0.02 µg per lane.

Anti-AKT1/2/3 antibody - ab139380 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of formalin-fixed, paraffin-embedded Human breast tissue labelling AKT1/2/3 with ab139380 at 10 &microg/ml.

Anti-AKT1/2/3 antibody - ab184136 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AKT1/2/3 antibody (ab184136) at 1/1000 dilutionLane 1 : 293T whole cell lysate/extractLane 2 : A431 whole cell lysate/extractLane 3 : HeLa whole cell lysate/extractLane 4 : HepG2 whole cell lysate/extractLane 5 : A375 whole cell lysate/extractLysates/proteins at 30 µg per lane.

Anti-AKT1/2/3 antibody [EPR16798] - ab179463 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16798
All lanes : Anti-AKT1/2/3 antibody [EPR16798] (ab179463) at 1/10000 dilutionLane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysatesLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysatesLane 3 : Hep G2 (Human liver hepatocellular carcinoma) whole cell lysatesLane 4 : A549 (Human lung carcinoma) whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Anti-AKT1/2/3 antibody [EPR16798] (Alexa Fluor® 488) - ab200619 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16798
ab200619 staining AKT1/2/3 in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200619 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-AKT1/2/3 antibody [EPR17671] - ab185633 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17671
All lanes : Anti-AKT1/2/3 antibody [EPR17671] (ab185633) at 1/5000 dilutionLane 1 : AKT3 recombinant protein fragment (His-Tag®): aa351-479Lane 2 : AKT2 recombinant protein fragment (His-Tag®): aa282-481Lane 3 : AKT1 recombinant protein fragment (His-Tag®): aa281-480Lysates/proteins at 0.01 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Anti-AKT1/2/3 antibody [EPR17737] - ab200195 from Abcam

Prices $347.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17737
All lanes : Anti-AKT1/2/3 antibody [EPR17737] (ab200195) at 1/2000 dilutionLane 1 : AKT3 recombinant protein fragment (His-Tag®): aa351-479Lane 2 : AKT2 recombinant protein fragment (His-Tag®): aa282-481Lane 3 : AKT1 recombinant protein fragment (His-Tag®): aa281-480Lysates/proteins at 0.01 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Anti-AKT1/3 (phospho Y473) antibody - ab138365 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-AKT1/3 (phospho Y473) antibody (ab138365) at 1/500 dilution + K562 cell extract treated with insulin 0.01U/ml for 15 min at 10 µg

Anti-AKT1/3 antibody - ab79361 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AKT1/3 antibody (ab79361) at 1/500 dilutionLane 1 : A549 cell extract Lane 2 : A549 cell extract with immunizing peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-AKT3 antibody - ab89430 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-AKT3 antibody (ab89430) at 5 µg/ml + AKT3 recombinant protein fragment at 0.2 µg

Anti-AKT3 antibody - ab189643 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling AKT3 with ab189643 at 10 µg/ml.

Anti-AKT3 antibody - ab5922 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AKT3 antibody (ab5922) at 0.5 µg/mlLane 1 : Lovo cell lysates.Lane 2 : Lovo cell lysates with Anti AKT3 peptide
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