Anti-AKT1/2/3 antibody [EPR17737]

• Supplier: Abcam
• Catalog: ab200195
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17737
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human AKT1/2/3 aa 400 to the C-terminus (Cysteine residue)
• Description: Rabbit Monoclonal
• Gene: AKT3
• Conjugate: Unconjugated

Product images

All lanes : Anti-AKT1/2/3 antibody [EPR17737] (ab200195) at 1/2000 dilutionLane 1 : AKT3 recombinant protein fragment (His-Tag®): aa351-479Lane 2 : AKT2 recombinant protein fragment (His-Tag®): aa282-481Lane 3 : AKT1 recombinant protein fragment (His-Tag®): aa281-480Lysates/proteins at 0.01 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Anti-AKT1/2/3 antibody [EPR17737] (ab200195) at 1/2000 dilution + MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-AKT1/2/3 antibody [EPR17737] (ab200195) at 1/10000 dilution + HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-AKT1/2/3 antibody [EPR17737] (ab200195) at 1/10000 dilutionLane 1 : PC-3 (Human prostate cancer cell line) whole cell lysateLane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLane 3 : 293 (Human embryonic kidney) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-AKT1/2/3 antibody [EPR17737] (ab200195) at 1/2000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human fetal heart lysateLane 3 : Human fetal kidney lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-AKT1/2/3 antibody [EPR17737] (ab200195) at 1/2000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse spleen lysateLane 3 : Rat brain lysateLane 4 : Rat heart lysateLane 5 : C6 (Rat glial tumor cells) whole cell lysateLane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling AKT1/2/3 with ab200195 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).Cytoplasm staining on MCF7 cell line is observed.The nuclear counter stain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab200195 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.