All lanes : Anti-AKT1 antibody [4D6] (ab124341) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY AKT1 cDNALysates/proteins at 5 µg per lane.
All lanes : Anti-AKT1 antibody [4D6] (ab124341) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : SVT2 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
ab124341 at 1/100 dilution staining AKT1 in COS7 cells transiently transfected with pCMV6-ENTRY AKT1 by Immunofluorescence.
ab124341 at 1/100 dilution staining AKT1 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
ab124341 at 1/150 dilution staining AKT1 in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
ab124341 at 1/150 dilution staining AKT1 in Paraffin-embedded Human kidney carcinoma tissue by Immunohistochemistry.
ab124341 at 1/150 dilution staining AKT1 in Paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
ab124341 at 1/150 dilution staining AKT1 in Paraffin-embedded Human prostate tissue by Immunohistochemistry.
ab124341 at 1/150 dilution staining AKT1 in Paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.