Anti-AKT1 antibody [Y89]
| Name | Anti-AKT1 antibody [Y89] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32505 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | Y89 |
| Applications | ICC/IF ICC/IF WB IHC-P FC IP |
| Species Reactivities | Mouse, Human, Rat, Bovine |
| Antigen | synthetic peptide corresponding to residues in C-terminus of human Akt1 |
| Description | Rabbit Monoclonal |
| Gene | AKT1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab32505 staining AKT1 in SK-N-SH cells treated with alsterpaullone (ab141070), by ICC/IF. Decrease of AKT1 expression correlates with increased concentration of alsterpaullone, as described in literature.The cells were incubated at 37°C for 6h in media containing different concentrations of ab141070 (alsterpaullone) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32505 (1/200 dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
![Anti-AKT1 antibody [Y89] (ab32505) at 1/10000 dilution + MCF-7 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/4453_ab32505.gif)
Anti-AKT1 antibody [Y89] (ab32505) at 1/10000 dilution + MCF-7 cell lysate
Immunohistochemical analysis of paraffin-embedded prostate carcinoma using ab32505 at 1/100 dilution.Immunohistochemical analysis of paraffin-embedded prostate carcinoma using ab32505 at 1/100 dilution.
Overlay histogram showing HeLa cells stained with ab32505 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32505, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
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