Anti-AKT2 antibody
| Name | Anti-AKT2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab39663 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P ELISA |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | The antiserum was produced against synthesized non phosphopeptide derived from human AKT2 around the phosphorylation site of serine 474 |
| Description | Rabbit Polyclonal |
| Gene | AKT2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab39663 staining human AKT2 in human lung carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
ICC/IF image of ab39663 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39663, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.