AKT2

Gene Symbol AKT2
Entrez Gene 208
Alt Symbol HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA
Species Human
Gene Type protein-coding
Description v-akt murine thymoma viral oncogene homolog 2
Other Description PKB beta|RAC-PK-beta|RAC-beta serine/threonine-protein kinase|murine thymoma viral (v-akt) homolog-2|protein kinase Akt-2|protein kinase B beta|rac protein kinase beta
Swissprots P31751 Q68GC0 Q0VAN1 Q0VAN0 Q05BV0 B2RBD8
Accessions AAT97984 EAW56941 EAW56942 EAW56943 P31751 AK054771 AK055779 BAG51573 AK122839 AK294453 BAG57690 AK304209 BAH14135 AK308006 AK308906 AK314619 BAG37185 BC022779 AAH22779 BC032709 AAH32709 BC040028 BC063421 AAH63421 BC120994 AAI20995 BC120995 AAI20996 BM675176 BQ219902 CN351354 M77198 AAA36585 M95936 AAA58364 XM_011526614 XP_011524916 XM_011526615 XP_011524917 XM_011526616 XP_011524918 XM_011526617 XP_011524919 XM_011526618 XP_011524920 XM_011526619 XP_011524921 XM_011526620 XP_011524922 XM_011526621 XP_011524923 XM_011526622 XP_011524924 NM_001243027 NP_001229956 NM_001243028 NP_001229957 NM_001626 NP_001617
Function AKT2 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glyco
Subcellular Location Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Note=Localizes within both nucleus and cytoplasm of proliferative primary myoblasts and mostly within the nucleus of differentiated primary myoblasts. By virtue of the N-terminal PH domain, is recruited to sites of the plasma membrane containing increased PI(3,4,5)P3 or PI(3,4)P2, cell membrane targeting is also facilitared by interaction with CLIP3.
Tissue Specificity Expressed in all cell types so far analyzed.
Top Pathways Apoptosis, Tight junction, VEGF signaling pathway, Focal adhesion, Toll-like receptor signaling pathway

Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb (Akt2 Specific) - 8599 from Cell Signaling Technology

Prices $307.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3H2
Western blot analysis of extracts from Akt1 (-/-) mouse embryonic fibroblasts (MEF) or Akt2 (-/-) MEF, untreated (-) or treated with Human Platelet-Derived Growth Factor AA (hPDGF-AA) #8913 (100 ng/ml, 15 min; +), using Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb (upper), Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb #4060 (middle), or Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

Akt2 (L79B2) Mouse mAb - 5239 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone L79B2
Western blot analysis of extracts from Akt1, Akt2 or Akt3 -/- mouse embryonic fibroblasts using Akt2 (L79B2) Mouse mAb (upper) and Akt1 (C73H10) Rabbit mAb #2938 (lower).

Akt2 (D6G4) Rabbit mAb - 3063 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6G4
Western blot analysis of extracts from various cell lines using Akt2 (D6G4) Rabbit mAb (upper) and Akt1 (C73H10) Rabbit mAb #2938 (lower).

Akt2 (5B5) Rabbit mAb - 2964 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 5B5
Western blot analysis of Akt1, Akt2 and Akt3 recombinant proteins, and extracts from Jurkat, C2C12, PC12 and COS cells, using Akt2 (5B5) Rabbit mAb.

Akt2 Antibody - 2962 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of immunoprecipitates from C6 cell lysates. Akt1 was immunoprecipitated with Akt1 (2H10) mAb #2967 (lane 1), Akt 2 was immunoprecipitated with Akt2 Antibody (lane 2), and specificity was confirmed by immunprecipitation with rabbit IgG (lane 3). Immunoblot was performed using Akt2 Antibody.

Akt2 (54G8) Rabbit mAb (IHC Specific) - 4057 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone 54G8

Akt2 (1G8C12) - sc-81148 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Akt2 (1G8C12): sc-81148. Western blot analysis of Akt2 expression in MCF7 (A), HeLa (B), A-431 (C) and THP-1 (D) whole cell lysates.

Akt2 (D-17) - sc-7127 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Akt2 (D-17): sc-7127. Western blot analysis of Akt2 expression in THP-1 whole cell lysate.

Akt2 (F-7) - sc-5270 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Akt2 (F-7): sc-5270. Western blot analysis of Akt2 expression in MCF7 whole cell lysate.

Akt2 (8B7) - sc-81436 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Akt2 (8B7): sc-81436. Western blot analysis of Akt2 expression in non-transfected 293T: sc-117752 (A), human Akt2 transfected 293T: sc-116831 (B) and A-431 (C) whole cell lysates.

p-Akt1/2/3 (Ser 473) - sc-135651 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Akt1/2/3 (Ser 473): sc-135651. Western blot analysis of phosphorylated Akt2 expression in untreated (A) TNF-a-treated (B) and UV-treated (C) A2780 cell extracts.

Anti-AKT2 (phospho S474) antibody - ab38513 from Abcam

Prices $380.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab38513 staining AKT2 (phospho S474) in 293T cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.1% Tween and blocked with 3% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/20 in PBS + 3% BSA) for 2 hours. An Alexa Fluor® 555-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview

Anti-AKT2 (phospho S474) antibody - ab97727 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab97727 at 1/50 dilution staining AKT2 in Human lung carcinoma by Immunohistochemistry, Paraffin-embedded tissue in the absence or presence of the immunising peptide.

Anti-AKT2 antibody - ab13991 from Abcam

Prices $391.00
Sizes 50 µg
Host Chicken
Clonality Polyclonal
Anti-AKT2 antibody (ab13991) at 1/500 dilution + mouse skin, whole tissue lysate at 4 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-AKT2 antibody - ab5920 from Abcam

Prices $379.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-AKT2 antibody - ab39663 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-AKT2 antibody - ab71045 from Abcam

Prices $378.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AKT2 antibody (ab71045) at 1/50 dilutionLane 1 : Non-transfected 293 cell lysatesLane 2 : AKT2 transfected 293 cell lysatesLysates/proteins at 2 µg per lane.

Anti-AKT2 antibody - ab9763 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-AKT2 antibody - ab54754 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-AKT2 antibody - ab70960 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-AKT2 antibody (ab70960) at 1/100 dilution + Jurkat cell lysate at 12.5 µgSecondaryHRP-anti-rabbit

Anti-AKT2 antibody - ab54757 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-AKT2 antibody [1B6] - ab130255 from Abcam

Host Mouse
Clonality Monoclonal
Clone 1B6
All lanes : Anti-AKT2 antibody [1B6] (ab130255) at 1/500 dilutionLane 1 : A431 cell lysateLane 2 : Jurkat cell lysateLane 3 : HEK293 cell lysateLane 4 : A549 cell lysateLane 5 : MCF7 cell lysateLane 6 : PC-12 cell lysate

Anti-AKT2 antibody [1G8C12] - ab47963 from Abcam

Host Mouse
Clonality Monoclonal
Clone 1G8C12
All lanes : Anti-AKT2 antibody [1G8C12] (ab47963) at 1/100 dilutionLane 1 : Truncated Akt2 recombinant proteinLane 2 : A431 cell lysate

Anti-AKT2 antibody [4H7] - ChIP Grade - ab175354 from Abcam

Prices $387.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4H7
Immunofluorescent analysis of AKT2 (green) showing staining in the cytoplasm and nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an AKT2 monoclonal antibody (ab175354) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Anti-AKT2 antibody [EP1676] - ab131168 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1676
All lanes : Anti-AKT2 antibody [EP1676] (ab131168) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit IgG at 1/2000 dilution
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