All lanes : Anti-ALDH4A1 antibody [EPR14288(B)] (ab181256) at 1/20000 dilutionLane 1 : Human fetal heart lysateLane 2 : HepG2 cell lysateLane 3 : HeLa cell lysateLane 4 : K562 cell lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Anti-ALDH4A1 antibody [EPR14288(B)] (ab181256) at 1/20000 dilution + Human fetal liver lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling ALDH4A1 with ab181256 at a 1/250 dilution. Counter stained hematoxylin.
Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling ALDH4A1 with ab181256 at a 1/250 dilution. Counter stained hematoxylin.
Immunofluorescence analysis of, paraformaldehyde-fixed, HepG2 cells labeling ALDH4A1 with ab181256 at a 1/1000 dilution. As secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) was used at a 1/200 dilution. In blue DAPI staining.
Western blot analysis on Immunoprecipitation from HepG2 cell lysate; showing ALDH4A1, using ab181256 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.