All lanes : Anti-ALIX antibody [EPR15314] - N-terminal (ab186429) at 1/5000 dilutionLane 1 : C6 cell lysateLane 2 : RAW 246.7 cell lysateLane 3 : PC12 cell lysateLane 4 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling ALIX with ab186429 at 1/240 dilution (red) or a rabbit IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
All lanes : Anti-ALIX antibody [EPR15314] - N-terminal (ab186429) at 1/5000 dilutionLane 1 : 293 cell lysateLane 2 : HeLa cell lysateLane 3 : K562 cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution