PDCD6IP

Gene Symbol PDCD6IP
Entrez Gene 10015
Alt Symbol AIP1, ALIX, DRIP4, HP95
Species Human
Gene Type protein-coding
Description programmed cell death 6 interacting protein
Other Description ALG-2 interacting protein 1|ALG-2-interacting protein X|PDCD6-interacting protein|apoptosis-linked gene 2-interacting protein X|dopamine receptor interacting protein 4|programmed cell death 6-interacting protein
Swissprots E9PFU1 Q9P2H2 Q9NUN0 C5MQH7 Q9UKL5 Q9BX86 Q6NUS1 Q8WUM4
Accessions EAW64468 EAW64469 Q8WUM4 AB037796 BAA92613 AF151793 AAF08220 AF250394 AAQ14260 AF349951 AAK20398 AI346371 AK002122 BAA92092 AK295042 BAG58093 AK298727 BAH12857 AL695629 BC020066 AAH20066 BC068454 AAH68454 BI492166 BI562417 BM669193 BQ024882 BQ895877 BT007367 AAP36031 BU165197 BU194828 CR739098 DA149360 DA652213 GQ131806 ACS12984 XM_011533252 XP_011531554 XM_011533253 XP_011531555 NM_001162429 NP_001155901 NM_001256192 NP_001243121 NM_013374 NP_037506 NR_027867
Function Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cel
Subcellular Location Cytoplasm, cytosol. Melanosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells.
Top Pathways Endocytosis

Alix (3A9) Mouse mAb - 2171 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 3A9
Western blot analysis of cell extracts from various cell types using Alix (3A9) Mouse mAb.

Alix (1A12) - sc-53540 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Alix (1A12): sc-53540. Western blot analysis of Alix expression in non-transfected: sc-110760 (A) and human Alix transfected: sc-113330 (B) 293 whole cell lysates.

Alix (C-11) - sc-271975 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Alix (C-11): sc-271975. Western blot analysis of Alix expression in non-transfected 293: sc-110760 (A), human Alix transfected 293: sc-113330 (B), Jurkat (C) and HeLa (D) whole cell lysates.

Alix (G-10) - sc-166952 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Alix (G-10): sc-166952. Western blot analysis of Alix expression in non-transfected: sc-110760 (A) and human Alix transfected: sc-113330 (B) 293 whole cell lysates.

Alix (H-270) - sc-99010 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Alix (H-270): sc-99010. Western blot analysis of Alix expression in U-937 (A), HeLa (D), Jurkat (E) and THP-1 (F) nuclear extracts.

Alix (N-20) - sc-49267 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Alix (N-20): sc-49267. Western blot analysis of Alix expression in non-transfected: sc-110760 (A) and human Alix transfected: sc-113330 (B) 293 whole cell lysates.

Alix (Q-19) - sc-49268 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Alix (Q-19): sc-49268. Western blot analysis of Alix expression in K-562 (A), U-937 (B), Jurkat (C) and THP-1 (D) nuclear extracts.

Alix (2H12) - sc-53539 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Alix (2H12): sc-53539. Western blot analysis of Alix expression in non-transfected: sc-110760 (A) and human Alix transfected: sc-113330 (B) 293 whole cell lysates.

Alix (3A9) - sc-53538 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Alix (3A9): sc-53538. Western blot analysis of Alix expression in non-transfected: sc-110760 (A) and human Alix transfected: sc-113330 (B) 293 whole cell lysates.

Anti-ALIX antibody - ab88743 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-ALIX antibody (ab88743) at 1 µg/ml + Human liver lysate at 50 µg

Anti-ALIX antibody - ab88388 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ALIX antibody (ab88388) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLane 4 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-ALIX antibody - ab50249 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALIX antibody - ab104256 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of PDC6I in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab104256 at 3 µg/mg lysate for IP (Lane 1). For WB detection an anti-PDC6I antibody which recognizes a downstream epitope was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-ALIX antibody [3A9] - ab117600 from Abcam

Prices $388.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 3A9
Anti-ALIX antibody [3A9] (ab117600) + HeLa whole cell lysateSecondaryRabbit Anti Mouse IgG

Anti-ALIX antibody [3C4] - ab56932 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3C4
Western blot against tagged recombinant protein immunogen using ab56932 PDC6I antibody at 1ug/ml.

Anti-ALIX antibody [8E8] - ab128526 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 8E8
All lanes : Anti-ALIX antibody [8E8] (ab128526) at 1/200 dilutionLane 1 : PDC6I recombinant protein at 0.005 µgLane 2 : PDC6I recombinant protein at 0.015 µgLane 3 : PDC6I recombinant protein at 0.05 µg

Anti-ALIX antibody [EPR15314] - N-terminal - ab186429 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR15314
All lanes : Anti-ALIX antibody [EPR15314] - N-terminal (ab186429) at 1/5000 dilutionLane 1 : C6 cell lysateLane 2 : RAW 246.7 cell lysateLane 3 : PC12 cell lysateLane 4 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-ALIX antibody [EPR15314-33] - N-terminal - ab186728 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR15314-33
All lanes : Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728) at 1/10000 dilutionLane 1 : 293 cell lysateLane 2 : K562 cell lysateLane 3 : Jurkat cell lysateLane 4 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-PDCD6IP / ALIX Antibody - LS-C44918 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-PDCD6IP / ALIX Antibody - LS-C121356 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-PDCD6IP / ALIX Antibody - LS-C144655 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PDCD6IP / ALIX Antibody (clone 8E8) - LS-C153291 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 8E8

Anti-PDCD6IP / ALIX Antibody (clone G1/G1-4) - LS-C189649 from LifeSpan Bioscience

Prices $335.00
Sizes 25 µg
Host Mouse
Clonality Monoclonal
Clone G1/G1-4

Anti-PDCD6IP / ALIX Antibody (aa541-570, AP) - LS-C238882 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PDCD6IP / ALIX Antibody (aa541-570, APC) - LS-C238883 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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