Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [EPR4477]

• Supplier: Abcam
• Catalog: ab108337
• Prices: $99.00
• Sizes: 10 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4477
• Applications: FC WB IP IHC-P ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Alkaline Phosphatase, Tissue Non-Specific aa 1-100
• Description: Rabbit Monoclonal
• Gene: ALPL
• Conjugate: Unconjugated

Product images

Overlay histogram showing Saos 2 cells stained with ab108337 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108337, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Saos 2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [EPR4477] (ab108337) at 1/10000 dilutionLane 1 : SAOS2 cell lysateLane 2 : HepG2 cell lysateLane 3 : HeLa cell lysateLane 4 : JAR cell lysateLysates/proteins at 10 µg per lane.

ab108337, at a 1/250 dilution, staining Alkaline Phosphatase, Tissue Non-Specific in paraffin embedded Human kidney tissue by Immunohistochemistry.

ab108337, at a 1/250 dilution, staining Alkaline Phosphatase, Tissue Non-Specific in paraffin embedded Human liver tissue by Immunohistochemistry.

ab108337, at a 1/100 dilution, staining Alkaline Phosphatase, Tissue Non-Specific in HeLa cells by Immunofluorescence.

Product References

Adhesion, vitality and osteogenic differentiation capacity of adipose derived - Adhesion, vitality and osteogenic differentiation capacity of adipose derived

Strauss S, Neumeister A, Barcikowski S, Kracht D, Kuhbier JW, Radtke C, Reimers K, Vogt PM. PLoS One. 2013;8(1):e53309.