ALPL

Gene Symbol ALPL
Entrez Gene 249
Alt Symbol AP-TNAP, APTNAP, HOPS, TNAP, TNSALP
Species Human
Gene Type protein-coding
Description alkaline phosphatase, liver/bone/kidney
Other Description alkaline phosphatase liver/bone/kidney isozyme|alkaline phosphatase, tissue-nonspecific isozyme|alkaline phosphomonoesterase|glycerophosphatase|liver/bone/kidney-type alkaline phosphatase|tissue-nonspecific ALP
Swissprots Q5BKZ5 O75090 B7Z387 Q6NZI8 Q59EJ7 Q9UBK0 P05186 Q8WU32 B7Z4Y6 Q2TAI7 B2RMP8 Q5VTG5 A1A4E7
Accessions AAB59378 AER29832 EAW94974 EAW94975 EAW94976 EAW94977 P05186 AB011406 BAA32129 AB012643 BAA88367 AB209814 BAD93051 AK097413 AK293184 BAH11467 AK295608 BAH12123 AK298085 BAH12722 AK312667 BAG35549 BC021289 AAH21289 BC066116 AAH66116 BC090861 AAH90861 BC110909 AAI10910 BC126165 AAI26166 BC136325 AAI36326 CN425768 DA574668 DA625627 DA631560 HQ258287 ADR83041 X95994 XM_005245818 XP_005245875 XM_005245820 XP_005245877 XM_006710546 XP_006710609 NM_000478 NP_000469 NM_001127501 NP_001120973 NM_001177520 NP_001170991
Function This isozyme may play a role in skeletal mineralization.
Subcellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Top Pathways Folate biosynthesis

TRA-2-54 (2J) Mouse mAb - 4747 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 2J
Western blot analysis of extracts from various cell lines using TRA-2-54 (2J) Mouse mAb.

Alkaline Phosphatase (H-300) - sc-30203 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Alkaline Phosphatase (H-300): sc-30203. Western blot analysis of TNAP expression in non-transfected: sc-117752 (A) and human TNAP transfected: sc-112494 (B) 293T whole cell lysates.

TNAP (3H414) - sc-73525 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TNAP (3H414): sc-73525. Western blot analysis of TNAP expression in non-transfected 293T: sc-117752 (A), human TNAP transfected 293T: sc-112384 (B) and NTERA-2 cl.D1 (C) whole cell lysates.

TNAP (3H415 ) - sc-73524 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TNAP (3H415 ): sc-73524. Western blot analysis of TNAP expression in non-transfected: sc-117752 (A) and human TNAP transfected: sc-112494 (B) 293T whole cell lysates.

TNAP (B4-78) - sc-81754 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TNAP (B4-78): sc-81754. Western blot analysis of TNAP expression in non-transfected: sc-117752 (A) and human TNAP transfected: sc-112494 (B) 293T whole cell lysates.

TNAP (N-18) - sc-23430 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

TNAP (TRA-2-39) - sc-21708 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TNAP (TRA-2-39): sc-21708. Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing membrane and cytoplasmic staining of glandular cells.

TNAP (TRA-2-54) - sc-21707 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TNAP (TRA-2-54): sc-21707. Indirect FCM analysis of live NTERA-2 cl.D1 cells stained with TNAP (TRA-2-54), followed by FITC-conjugated goat anti-mouse IgG<sub>1</sub>: sc-2078.

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody - ab65834 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
This image shows ab65834 at 1-2µg/ml staining cytoplasmic Alkaline Phosphatase in rat small intestine tissue.

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody - ab86789 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab86789) at 1 µg/ml + Kidney (Human) Tissue Lysate - adult normal tissue (ab30203) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody - ab135972 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab135972) at 1/500 dilution + Jurkat cell lysate at 30 µg

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] - ab126820 from Abcam

Prices $387.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2F4
ICC/IF image of ab126820 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab126820 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) hepG2 cells used at 1/100 dilution.

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [3H414 (TRA-2-49)] - ab17973 from Abcam

Prices $384.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 3H414 (TRA-2-49)
Undifferentiated human embryonic stem cells (H9 line) were stained with the tissue non-specific alkaline phosphatase antibody, ab17973. The cells were positive for alkaline phosphatase with staining localised on the surface membrane.Dilution used: 1/50Note that the feeder cells in the bottom left hand corner of the plate are not stained by this antibody.

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [3H415 (TRA-2-54)] - ab17989 from Abcam

Prices $379.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 3H415 (TRA-2-54)
Undifferentiated human embryonic stem cells (H9 line) were stained with the tissue non-specific alkaline phosphatase antibody, ab17973. The cells were positive for alkaline phosphatase with staining localised on the surface membrane.Dilution used: 1/50

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [EPR4477] - ab108337 from Abcam

Prices $99.00
Sizes 10 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4477
Overlay histogram showing Saos 2 cells stained with ab108337 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108337, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Saos 2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Bone Alkaline Phosphatase antibody - ab68716 from Abcam

Prices $370.00
Sizes 500 µl
Host Sheep
Clonality Polyclonal

Anti-ALPL / Alkaline Phosphatase Antibody (aa10-40, AP) - LS-C280797 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALPL / Alkaline Phosphatase Antibody - LS-C192354 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of ALPL pAb in extracts from HepG2 and Hela cells.

Anti-ALPL / Alkaline Phosphatase Antibody (aa10-40, APC) - LS-C280798 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALPL / Alkaline Phosphatase Antibody (aa10-40, PE) - LS-C280799 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALPL / Alkaline Phosphatase Antibody - LS-C285770 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ALPL / Alkaline Phosphatase Antibody (aa18-335, Biotin) - LS-C297956 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ALPL / Alkaline Phosphatase Antibody - LS-C306222 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-ALPL / Alkaline Phosphatase Antibody (aa18-335, FITC) - LS-C302770 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ALPL / Alkaline Phosphatase Antibody (Internal, clone EPR4477) IHC-plus™ - LS-B6663 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4477
Anti-Bone Alkaline Phosphatase antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6663 dilution 1:50.
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