Anti-alpha + beta Synuclein antibody [EP1646Y]

• Supplier: Abcam
• Catalog: ab51252
• Prices: $391.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1646Y
• Applications: FC WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human alpha Synuclein aa 1-100 (N terminal)
• Description: Rabbit Monoclonal
• Gene: SNCB
• Conjugate: Unconjugated

Product images

Overlay histogram showing SH-SY5Y cells stained with ab51252 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51252, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252) at 1/50000 dilution + Fetal brain lysate at 10 µgSecondaryGoat anti-Rabbit HRP labeled. at 1/2000 dilution

Immunofluorescent staining of PC12 cells using ab51252 (1:50).

Immunofluorescence analysis of rat primary hippocampal neurons, staining alpha Synuclein with ab51252.Cells were fixed, permeabilized, and blocked with 10% donkey serum at room temperature. Cells were incubated with primary antibody (1/1000) at 4°C for 24 hours. A Cy2-conjugated donkey anti-rabbit IgG was used as the secondary antibody.

Product References

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Elucidating the role of C-terminal post-translational modifications using protein - Elucidating the role of C-terminal post-translational modifications using protein

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Bacterial in-cell NMR of human alpha-synuclein: a disordered monomer by nature? - Bacterial in-cell NMR of human alpha-synuclein: a disordered monomer by nature?

Binolfi A, Theillet FX, Selenko P. Biochem Soc Trans. 2012 Oct;40(5):950-4.

Neuromelanin enhances the toxicity of alpha-synuclein in SK-N-SH cells. - Neuromelanin enhances the toxicity of alpha-synuclein in SK-N-SH cells.

Li J, Yang J, Zhao P, Li S, Zhang R, Zhang X, Liu D, Zhang B. J Neural Transm. 2012 Jun;119(6):685-91.

Lrrk2 phosphorylates alpha synuclein at serine 129: Parkinson disease - Lrrk2 phosphorylates alpha synuclein at serine 129: Parkinson disease

Qing H, Wong W, McGeer EG, McGeer PL. Biochem Biophys Res Commun. 2009 Sep 11;387(1):149-52. doi: