Anti-alpha 2 Macroglobulin antibody
| Name | Anti-alpha 2 Macroglobulin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58703 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA IHC-P ICC/IF |
| Species Reactivities | Rat, Human, Mouse |
| Antigen | Synthetic peptide corresponding to C terminal residues of human alpha 2 Macroglobulin |
| Description | Rabbit Polyclonal |
| Gene | A2M |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab58703 staining alpha 2 Macroglobulin in human liver.Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab58703 staining alpha 2 Macroglobulin in rat decidual cells by Immunocytochemistry. Cells were fixed with Methanol, permeabilized with PBS, 5% BSA, 0.1% Triton X-100 and 0.2% Tween-20 and blocking with 5% BSA was performed for 15 minutes at RT. Samples were incubated with primary antibody (1/500) in PBS for 14 hours at 4°C. A commercially available Avidin–biotin alkaline phosphatase complex technique was used.See Abreview
All lanes : Anti-alpha 2 Macroglobulin antibody (ab58703) at 1/1000 dilutionLane 1 : lysates prepared from rat spinal cord tissues (control group)Lane 2 : lysates prepared from rat spinal cord tissues (control group)Lane 3 : lysates prepared from rat spinal cord tissues (control group)Lane 4 : lysates prepared from rat spinal cord tissues (encephalomyelitis group)Lane 5 : lysates prepared from rat spinal cord tissues (encephalomyelitis group)Lane 6 : lysates prepared from rat spinal cord tissues (encephalomyelitis group)Lysates/proteins at 30 µg per lane.Secondarygoat anti-rabbit IgG coupled to horseradish peroxidase
Anti-alpha 2 Macroglobulin antibody (ab58703) at 1 µg/ml + Human alpha 2 Macroglobulin full length protein (ab77935) at 0.1 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 3 minutes
Product References
Nasal mucus proteomic changes reflect altered immune responses and epithelial - Nasal mucus proteomic changes reflect altered immune responses and epithelial
Tomazic PV, Birner-Gruenberger R, Leitner A, Obrist B, Spoerk S, Lang-Loidolt D. J Allergy Clin Immunol. 2014 Mar;133(3):741-50.
Altered proteolytic events in experimental autoimmune encephalomyelitis - Altered proteolytic events in experimental autoimmune encephalomyelitis
Jain MR, Bian S, Liu T, Hu J, Elkabes S, Li H. Proteome Sci. 2009 Jul 16;7:25.
Anandamide-induced cell death: dual effects in primary rat decidual cell - Anandamide-induced cell death: dual effects in primary rat decidual cell
Fonseca BM, Correia-da-Silva G, Teixeira NA. Placenta. 2009 Aug;30(8):686-92.