A2M

Gene Symbol A2M
Entrez Gene 2
Alt Symbol A2MD, CPAMD5, FWP007, S863-7
Species Human
Gene Type protein-coding
Description alpha-2-macroglobulin
Other Description C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5|alpha-2-M
Swissprots Q13677 Q68DN2 P01023 Q59F47 Q5QTS0 Q6PN97 Q6PIY3
Accessions AAK38109 AAK38110 CAA48670 CAA77774 CCO13665 EAW88590 P01023 AB209614 BAD92851 AK307832 AK312482 AY591530 AAT02228 BC026246 AAH26246 BC040071 AAH40071 BX504041 BX647329 BX648622 CR749334 CAH18188 M11313 AAA51551 M36501 AAA51552 XM_006719056 XP_006719119 NM_000014 NP_000005
Function Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Subcellular Location Secreted {ECO:0000269|PubMed:6203908}.
Tissue Specificity Secreted in plasma. {ECO:0000269|PubMed:6203908}.
Top Pathways Complement and coagulation cascades

α-2M (H-8) - sc-390544 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
α-2M (H-8): sc-390544. Western blot analysis of α-2M in human.

α-2M (H-60) - sc-28870 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
α-2M (H-60): sc-28870. Western blot analysis of human recombinant α-2M fusion protein.

α-2M (C-18) - sc-8514 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
α-2M (C-18): sc-8514. Western blot analysis of α-2M in human.

α-2M (R-19) - sc-8517 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
α-2M (R-19)-R: sc-8517-R. Western blot analysis of α-2M expression in non-transfected: sc-117752 (A) and human α-2M transfected: sc-115474 (B) 293T whole cell lysates.

α-2M (2D9) - sc-69750 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Immunoprecipitation of α-2M from human plasma (B) using α-2M (3D1)): sc-81540 (mouse monoclonal antibody) followed by Western blot analysis using α-2M (2D9): sc-69750 (mouse monoclonal antibody) as compared with human plasm (A).

α-2M (3D1) - sc-81540 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Immunoprecipitation of α-2M from human plasma (B) using α-2M (3D1)): sc-81540 (mouse monoclonal antibody) followed by Western blot analysis using α-2M (2D9): sc-69750 (mouse monoclonal antibody) as compared with human plasm (A).

α-2M (9A3) - sc-81541 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Immunoprecipitation of α-2M from human plasma (B) using α-2M (9A3): sc-81541 (mouse monoclonal antibody) followed by Western blot analysis using α-2M (2D9): sc-69750 (mouse monoclonal antibody) as compared with human plasma (A).

Anti-alpha 2 Macroglobulin antibody - ab58703 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Ab58703 staining alpha 2 Macroglobulin in human liver.Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-alpha 2 Macroglobulin antibody - ab7337 from Abcam

Prices $359.00
Sizes 5 mg
Host Goat
Clonality Polyclonal
ICC/IF image of ab7337 stained Malme-3m cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7337, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96931, DyLight® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-alpha 2 Macroglobulin antibody - ab48555 from Abcam

Prices $359.00
Sizes 200 µg
Host Rabbit
Clonality Polyclonal
Anti-alpha 2 Macroglobulin antibody (ab48555) at 1 µg/ml + Placenta (Human) Tissue Lysate - adult normal tissue (ab29745) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Anti-alpha 2 Macroglobulin antibody - ab181656 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-alpha 2 Macroglobulin antibody (ab181656) at 1/1000 dilution + Human alpha 2 Macroglobulin at 0.05 µgSecondaryPeroxidase anti-Goat at 1/40000 dilution

Anti-alpha 2 Macroglobulin antibody - ab5722 from Abcam

Host Chicken
Clonality Polyclonal

Anti-alpha 2 Macroglobulin antibody - ab56005 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-alpha 2 Macroglobulin antibody [2D9] - ab36995 from Abcam

Prices $392.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2D9
All lanes : Anti-alpha 2 Macroglobulin antibody [2D9] (ab36995)Lane 1 : Alpha 2 Macroglobulin isolated from Human plasmaLane 2 : Human plasmaLysates/proteins at 50 µg per lane.Secondaryanti-mouse IgG -HRP

Anti-alpha 2 Macroglobulin antibody (Biotin) - ab7338 from Abcam

Prices $359.00
Sizes 1 mg
Host Goat
Clonality Polyclonal

Anti-alpha 2 Macroglobulin antibody [EPR4431] - ab133299 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4431
Anti-alpha 2 Macroglobulin antibody [EPR4431] (ab133299) at 1/1000 dilution + Human plasma lysate at 10 µgSecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Anti-alpha 2 Macroglobulin antibody [EPR4432] - ab109422 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4432
Anti-alpha 2 Macroglobulin antibody [EPR4432] (ab109422) at 1/1000 dilution + Human plasma lysate at 10 µg

Anti-alpha 2 Macroglobulin antibody [EPSISR39] - ab156015 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPSISR39
All lanes : Anti-alpha 2 Macroglobulin antibody [EPSISR39] (ab156015) at 1/10000 dilutionLane 1 : Human serum lysateLane 2 : Human plasma lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-alpha 2 Macroglobulin antibody [F1-P1C11 3] - ab116593 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone F1-P1C11 3

Anti-alpha 2 Macroglobulin antibody (HRP) - ab7339 from Abcam

Prices $372.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-alpha 2 Macroglobulin antibody (HRP) - ab181647 from Abcam

Prices $372.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-CINAP antibody - ab122894 from Abcam

Prices $407.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
ab122894, at 5 µg/ml, staining CINAP in Formalin-fixed, Paraffin-embedded Human Testis tissue by Immunohistochemistry.

Anti-Dengue Virus 2 antibody - ab155042 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Dengue Virus 2 antibody (ab155042) at 1/1000 dilutionLane 1 : BHK-21 Lane 2 : Dengue Virus 2 infected BHK-21 Lysates/proteins at 20 µg per lane.

Anti-SCLY antibody - C-terminal - ab190983 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SCLY antibody - C-terminal (ab190983) at 0.5 µg/mlLane 1 : U87 cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLane 4 : MCF-7 cell lysateLane 5 : COLO320 cell lysateLane 6 : HE1080 cell lysate

Anti-A2M / Alpha-2-Macroglobulin Antibody (aa1161-1192, Azide-free, HRP) - LS-C212553 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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