Anti-alpha Actinin antibody [0.T.02]

• Supplier: Abcam
• Catalog: ab18061
• Prices: $394.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 0.T.02
• Applications: WB IHC-F IHC IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Sheep, Rabbit, Human
• Antigen: Human alpha Actinin
• Description: Mouse Monoclonal
• Gene: ACTN1
• Conjugate: Unconjugated

Product images

Anti-alpha Actinin antibody [0.T.02] (ab18061) at 1/2000 dilution + Human pancreatic cancer cell line - whole cell lysate at 20 µgSecondaryHRP conjugated goat anti-mouse antibody at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ICC/IF image of ab18061 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18061, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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