ACTN1

Gene Symbol ACTN1
Entrez Gene 87
Alt Symbol BDPLT15
Species Human
Gene Type protein-coding
Description actinin, alpha 1
Other Description F-actin cross-linking protein|actinin 1 smooth muscle|alpha-actinin cytoskeletal isoform|alpha-actinin-1|non-muscle alpha-actinin-1
Swissprots Q1HE25 P12814 B4DHH3 B7TY16 B3V8S3 Q9BTN1
Accessions EAW80971 EAW80972 EAW80973 EAW80974 EAW80975 EAW80976 P12814 AJ844628 CAH59747 AK098203 BAG53591 AK124378 AK225782 AK294317 BAG57591 AK295099 BAG58135 AK298496 BAH12801 AK299362 BAG61357 BC003576 AAH03576 BC004984 BC011987 BC015766 AAH15766 BC127124 AAI27125 BI494856 BT007207 AAP35871 BX248020 CAD62344 BX641076 DC321454 DC372566 DQ496098 ABF50047 DQ890778 ABM81704 DQ895768 ABM86694 EU716325 ACE62922 FJ410030 ACJ24535 M95178 AAA51582 X15804 CAA33803 X55187 CAA38970 XM_011537265 XP_011535567 XM_011537266 XP_011535568 XM_011537267 XP_011535569 XM_011537268 XP_011535570 XM_011537269 XP_011535571 XM_011537270 XP_011535572 XM_011537271 XP_011535573 NM_001102 NP_001093 NM_001130004 NP_001123476 NM_001130005 NP_001123477
Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Subcellular Location Cytoplasm, cytoskeleton {ECO:0000269|PubMed:24069336, ECO:0000269|PubMed:7750553}. Cytoplasm, myofibril, sarcomere, Z line {ECO:0000269|PubMed:7750553}. Cell membrane {ECO:0000250}. Cell junction {ECO:0000250}. Cell projection, ruffle {ECO:0000250}. Note=Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures (By similarity). {ECO:0000250}.
Top Pathways Systemic lupus erythematosus, Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Leukocyte transendothelial migration, Amoebiasis

α-Actinin (D6F6) XP ® Rabbit mAb (HRP Conjugate) - 12413 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6F6
Western blot analysis of extracts from various cell lines using α-Actinin (D6F6) XP ® Rabbit mAb (HRP Conjugate).

α-Actinin (D6F6) XP ® Rabbit mAb - 6487 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6F6
Western blot analysis of extracts from various cell lines using α-Actinin (D6F6) XP ® Rabbit mAb.

α-Actinin Antibody - 3134 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3, C6, CHO and COS cells using α-Actinin Antibody.

α-actinin (H-2) - sc-17829 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
tuberin siRNA (h): sc-36762. Western blot analysis of tuberin expression in non-transfected control (A) and tuberin siRNA transfected (B) HeLa cells. Blot probed with tuberin (C-20): sc-893. α-actinin (H-2): sc-17829 used as specificity and loading control.

α-actinin-1 (23) - sc-135819 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
α-actinin-1 (23): sc-135819. Western blot analysis of α-actinin-1 expression in Jurkat whole cell lysate.

Anti-alpha Actinin antibody - ab199290 from Abcam

Prices $376.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-alpha Actinin antibody (ab199290) at 1/1000 dilution + HeLa cell lysate

Anti-alpha Actinin antibody [0.T.02] - ab18061 from Abcam

Prices $394.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 0.T.02
Anti-alpha Actinin antibody [0.T.02] (ab18061) at 1/2000 dilution + Human pancreatic cancer cell line - whole cell lysate at 20 µgSecondaryHRP conjugated goat anti-mouse antibody at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-alpha Actinin antibody [7A4] - ab90421 from Abcam

Prices $385.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 7A4
Anti-alpha Actinin antibody [7A4] (ab90421) at 1/2000 dilution + recombinant human alpha Actinin

Anti-alpha Actinin antibody [AT6.172] - ab78505 from Abcam

Prices $359.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone AT6.172
ab78505 staining alpha Actinin in methanol fixed fibroblast.

Anti-alpha Actinin antibody [EP2527Y] - ab68194 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2527Y
Overlay histogram showing HeLa cells stained with ab68194 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab68194, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (IgG; H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-alpha Actinin antibody [EP2528Y] - ab81265 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2528Y
Overlay histogram showing HeLa cells stained with ab81265 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81265, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (IgG; H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-alpha Actinin antibody [SA-20] - ab82247 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone SA-20
Overlay histogram showing HeLa cells stained with ab82247 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82247, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-ACTN1 Antibody - LS-C34853 from LifeSpan Bioscience

Prices $560.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal

Anti-ACTN1 Antibody (N-Terminus) - LS-C34858 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-ACTN1 Antibody - LS-C121110 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-ACTN1 Antibody (clone CB11) - LS-C122583 from LifeSpan Bioscience

Prices $650.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone CB11

Anti-ACTN1 Antibody (C-Terminus) - LS-C126580 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ACTN1 Antibody - LS-C126581 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-ACTN1 Antibody (N-Terminus, AP) - LS-C274856 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ACTN1 Antibody (C-Terminus, clone EP2528Y) - LS-C137690 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2528Y
Western blot analysis on (A) NIH 3T3 (B) human skeletal muscle tissue (C) human heart tissue lysates using anti-alpha-actinin 1 antibody, dilution 1:1000.

Anti-ACTN1 Antibody (N-Terminus) - LS-C102772 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ACTN1 / ASMA antibody LS-C102772 Western blot of 293T cell lysate.

Anti-ACTN1 Antibody (N-Terminus, clone EP2527Y) - LS-C105275 from LifeSpan Bioscience

Prices $480.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2527Y
Western blot analysis on (A) NIH 3T3 (B) HeLa (C) C6 lysates using anti-alpha-actinin 1 antibody (LS-C105275), dilution 1:10000.

Anti-ACTN1 Antibody - LS-C183484 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ACTN1 Antibody (N-Terminus, Biotin) - LS-C274857 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ACTN1 Antibody (N-Terminus, Azide-free, HRP) - LS-C274859 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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