Anti-alpha Actinin antibody [EP2528Y]

• Supplier: Abcam
• Catalog: ab81265
• Prices: $379.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP2528Y
• Applications: WB ICC/IF ICC/IF FC IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues on the C terminal of human alpha Actinin
• Description: Rabbit Monoclonal
• Gene: ACTN1
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab81265 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81265, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (IgG; H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-alpha Actinin antibody [EP2528Y] (ab81265) at 1/1000 dilutionLane 1 : NIH 3T3 cell lysateLane 2 : human skeletal muscle tissue lysateLane 3 : human heart tissue lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution