Anti-Alpha B Crystallin antibody [EPR2752]

• Supplier: Abcam
• Catalog: ab76467
• Prices: $379.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR2752
• Applications: WB IHC-P ICC/IF ICC/IF IP
• Species Reactivities: Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Alpha B Crystallin (C terminal)
• Description: Rabbit Monoclonal
• Gene: CRYAB
• Conjugate: Unconjugated

Product images

Immunohistochemical staining of paraffin embedded human brain with purified ab76467 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/1000 dilution (purified) + Rat eyeball lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/1000 dilution (purified) + Human fetal heart tissue lysate at 10 µg/mlSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Immunofluorescence staining of HeLa cells with purified ab76467 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.

Immunohistochemical staining of paraffin embedded human brain with unpurified ab76467 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Immunohistochemical staining of alpha B Crystallin in paraffin embedded human heart muscle with ab76467 at a 1/100 dilution.

Lane 1 : Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/5000 dilutionLane 2 : Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/20000 dilutionLane 1 : fetal heart lysateLane 2 : rat eyeball lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

ab76467 (unpurified) at 1/250 immunoprecipitating alpha B crystallin in human fetal heart (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.