Anti-alpha Defensin NP5 antibody [8C8]
| Name | Anti-alpha Defensin NP5 antibody [8C8] |
|---|---|
| Supplier | Abcam |
| Catalog | ab62757 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 8C8 |
| Applications | IHC-P ICC/IF ICC/IF WB |
| Species Reactivities | Human |
| Antigen | Full length alpha Defensin NP5 propeptide |
| Description | Mouse Monoclonal |
| Gene | DEFA5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of Defensin NP5 staining in human duodenum formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab62757, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Altered expression of innate immunity genes in different intestinal sites of - Altered expression of innate immunity genes in different intestinal sites of
Stronati L, Negroni A, Pierdomenico M, D'Ottavio C, Tirindelli D, Di Nardo G, Oliva S, Viola F, Cucchiara S. Dig Liver Dis. 2010 Dec;42(12):848-53.
Mucosal gene expression of antimicrobial peptides in inflammatory bowel disease - Mucosal gene expression of antimicrobial peptides in inflammatory bowel disease
Arijs I, De Hertogh G, Lemaire K, Quintens R, Van Lommel L, Van Steen K, Leemans P, Cleynen I, Van Assche G, Vermeire S, Geboes K, Schuit F, Rutgeerts P. PLoS One. 2009 Nov 24;4(11):e7984.
Paneth cell antimicrobial peptides: topographical distribution and quantification - Paneth cell antimicrobial peptides: topographical distribution and quantification
Wehkamp J, Chu H, Shen B, Feathers RW, Kays RJ, Lee SK, Bevins CL. FEBS Lett. 2006 Oct 2;580(22):5344-50. Epub 2006 Sep 12.
Reduced Paneth cell alpha-defensins in ileal Crohn's disease. - Reduced Paneth cell alpha-defensins in ileal Crohn's disease.
Wehkamp J, Salzman NH, Porter E, Nuding S, Weichenthal M, Petras RE, Shen B, Schaeffeler E, Schwab M, Linzmeier R, Feathers RW, Chu H, Lima H Jr, Fellermann K, Ganz T, Stange EF, Bevins CL. Proc Natl Acad Sci U S A. 2005 Dec 13;102(50):18129-34. Epub 2005 Dec 5.