Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254) at 1/10000 dilution + Human skeletal muscle lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254) at 1/1000 dilutionLane 1 : Mouse heart lysateLane 2 : Rat heart lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunhistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling alpha Sarcoglycan with ab189254 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunoprecipitation analysis of human skeletal muscle tissue lysate labeling alpha Sarcoglycan using ab189254 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of human skeletal muscle tissue lysate.