Anti-alpha smooth muscle Actin antibody
| Name | Anti-alpha smooth muscle Actin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab5694 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F ICC/IF ICC/IF ICC/IF WB ELISA IHC-P IHC-F |
| Species Reactivities | Mouse, Rat, Chicken, Guinea Pig, Bovine, Dog, Human, Pig |
| Antigen | Synthetic peptide corresponding to Human alpha smooth muscle Actin |
| Description | Rabbit Polyclonal |
| Gene | ACTA2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Human Leiomyoma stained with ab5694.
This picture shows formalin-fixed, paraffin embedded mouse intestine and mesentery, the optimal dilution is 1:1600 to 1:3200, incubation overnight at 4oC, counterstained with Hematoxylin. This image was kindly supplied as part of the review by JQ Zhang.
All lanes : Anti-alpha smooth muscle Actin antibody (ab5694) at 1 µg/mlLane 1 : HeLa NuclearLane 2 : HeLa whole cellLane 3 : A431 cell lysateLane 4 : Jurkat cell lysateLane 5 : HEK293 cell lysateLysates/proteins at 20 µg per lane.SecondaryAlexa Fluor anti-rabbit at 1/5000 dilutionPerformed under reducing conditions.
Ab5694 positively staining smooth muscle cells in blood vessels and myoepithelial cells in the frozen tissue of cancerous human mammary gland (pink) at 1/100 dilution. Secondary: CY5 conjugated goat anti rabbit (1/100). Co immunostaining of glandular cell cytokeratin can be seen stained by FITC (green). Auto fluorescent erythrocytes that are present within blood vessels are shown (red), whilst the DAPI counter stain may clearly be seen staining nuclei (blue). This image is courtesy of an Abreview submitted by on 22 August 2005. We do not have any further information relating to this image. See Abreview
All lanes : Anti-alpha smooth muscle Actin antibody (ab5694) at 1/500 dilutionLane 1 : Rat2 myofibroblasts (untreated before treatment-0 days)Lane 2 : Rat2 myofibroblasts (untreated for 5 days)Lane 3 : Rat2 myofibroblasts (treated with 1ng/mL TGF beta)Lane 4 : Rat2 myofibroblasts (treated with 10ng/mL TGF beta)Lane 5 : Positive control (NIH3T3)Lane 6 : Negative control (MDA-MB-469 breast carcinoma cells)Lysates/proteins at 10 µg per lane.SecondaryDonkey anti rabbit (HRP) at 1/2500 dilutionPerformed under reducing conditions.
ab5694 at 1/500 staining rat myofibroblast cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 2 hours. A FITC conjugated goat anti-rabbit IgG was used as the secondary. Nuclei were counterstained with propidium iodide.See Abreview
ab5694 staining Human fetal heart cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.See Abreview
All lanes : Anti-alpha smooth muscle Actin antibody (ab5694) at 1/1000 dilutionLane 1 : Lystates prepared from pig heart tissue from normal control animalsLane 2 : Lystates prepared from pig heart tissue from normal control animalsLane 3 : Lystates prepared from pig heart tissue from experimental animalsLane 4 : Lystates prepared from pig heart tissue from experimental animalsLysates/proteins at 4 µg per lane.SecondaryHRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilutionPerformed under reducing conditions.
ab5694 staining alpha smooth muscle Actin in human skin tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocked with 10% serum for 1 hour at RT. The primary antibody was diluted 1/300 and incubated with sample in 2% serum for 15 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1/500 as secondary antibody.See Abreview
ab5694 staining alpha smooth muscle Actin in rat lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then permeabilized using 0.5 Triton X-100 for 20 minutes, blocked with 1% BSA for 30 minutes at 20°C and then incubated with ab5694 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 568 conjugated goat anti-rabbit polyclonal used at a 1/250 dilution.See Abreview
Product References
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