ACTA2

Gene Symbol ACTA2
Entrez Gene 423787
Alt Symbol asma
Species Chicken
Gene Type protein-coding
Description actin, alpha 2, smooth muscle, aorta
Other Description actin, aortic smooth muscle|alpha-actin
Swissprots P08023
Accessions AAA48571 AAB12010 P08023 CR389634 CR391391 NM_001031229 NP_001026400
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subcellular Location Cytoplasm, cytoskeleton.
Top Pathways Vascular smooth muscle contraction

Anti-alpha smooth muscle Actin antibody - ab5694 from Abcam

Prices $401.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Human Leiomyoma stained with ab5694.

Anti-alpha smooth muscle Actin antibody - ab15734 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-alpha smooth muscle Actin antibody (ab15734) at 1 µg/mlLane 1 : HeLa whole cell lysateLane 2 : A431 cell lysateLane 3 : HeLa whole cell lysate with Human alpha smooth muscle Actin peptide (ab16102) at 1 µg/mlLane 4 : A431 cell lysate with Human alpha smooth muscle Actin peptide (ab16102) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat-anti-Rabbit IgG at 1/10000 dilutionPerformed under reducing conditions.

Anti-alpha smooth muscle Actin antibody - ab137734 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-alpha smooth muscle Actin antibody (ab137734) at 1/1000 dilution + Molt4 whole cell lysate at 30 µg

Anti-alpha smooth muscle Actin antibody - ab139370 from Abcam

Prices $376.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labelling alpha smooth muscle Actin with ab139370 at 2.5 &microg/ml.

Anti-alpha smooth muscle Actin antibody [0.N.5] - ab18147 from Abcam

Prices $392.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone 0.N.5
ab18147 staining alpha smooth muscle actin in mouse muscle tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 10% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 18 hours at 4°C. An undiluted Phycoerythrin-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.See Abreview

Anti-alpha smooth muscle Actin antibody [1A4] (Alexa Fluor® 488) - ab184675 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1A4
Overlay histogram showing SV40LT-SMC cells stained with ab184675 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab184675, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in SV40LT-SMC fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-alpha smooth muscle Actin antibody [1A4] (Alexa Fluor® 594) - ab202368 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1A4
ab202368 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202368 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in SV40LT-SMC cells fixed with 100% methanol (5min).

Anti-alpha smooth muscle Actin antibody [1A4] (FITC) - ab8211 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1A4
ICC/IF image of ab8211 stained SV40LT-SMC cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (FITC conjugated ab8211, 10µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-alpha smooth muscle Actin antibody (Biotin) - ab188497 from Abcam

Prices $372.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-alpha smooth muscle Actin antibody (FITC) - ab188498 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-alpha smooth muscle Actin antibody, prediluted - ab15267 from Abcam

Prices $392.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
ab15267 staining alpha smooth muscle Actin in human leiomyoma by Immunohistochemistry (FFPE-sections).

Anti-alpha smooth muscle Actin antibody [SP171] - ab150301 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone SP171
Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon tissue labelling alpha smooth muscle Actin with ab150301 at 1/200 dilution.

Anti-smooth muscle Actin antibody [B4] - ab40863 from Abcam

Host Mouse
Clonality Monoclonal
Clone B4
Mouse detrusor muscle (layer of the urinary bladder wall made of smooth muscle fibers) detected with ab40863 used at 1/500 fixed in paraformaldehye and embedded in paraffin.

Monoclonal Anti-Actin, Smooth Muscle antibody produced in mouse - A7607 from Sigma-Aldrich

Prices $389.50
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone CGA7
<B>Immunofluorescence</B><BR/>Mouse colon sections, paraffin embedded-ethanol fixed were stained with 10 μg/mL of Monoclonal Anti-Actin, smooth Muscle Specific (<B>Cat. No. A7607</B>). The antibody was developed with Goat Anti-Mouse IgG, FITC conjugate (<B>Cat. No. F8771</B>).

Smooth Muscle Actin Monoclonal Antibody (a-SM1) - MA1-12772 from Thermo Fisher Scientific

Prices $425.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone a-SM1

JLA20 - JLA20 from Developmental Studies Hybridoma Bank

Host Mouse
Clonality Monoclonal
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