Anti-alpha smooth muscle Actin antibody [1A4] (Alexa Fluor® 488)
| Name | Anti-alpha smooth muscle Actin antibody [1A4] (Alexa Fluor® 488) |
|---|---|
| Supplier | Abcam |
| Catalog | ab184675 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | 1A4 |
| Applications | FC ICC/IF ICC/IF |
| Species Reactivities | Rat, Mouse, Sheep, Goat, Chicken, Guinea Pig, Cat, Dog, Human, Snake, Zebrafish |
| Description | Mouse Monoclonal |
| Gene | ACTA2 |
| Conjugate | Alexa Fluor® 488 |
| Supplier Page | Shop |
Product images
Overlay histogram showing SV40LT-SMC cells stained with ab184675 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab184675, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in SV40LT-SMC fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab184675 stained SV40LT-SMC cells. The cells were 100% Methanol fixed for 5 minutes at room temperature and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab184675 at 1/200) overnight at +4°C. The secondary antibody (pseudo-colored green) was a Goat Anti-Mouse Alexa Fluor® 488(IgG H&L) preadsorbed (ab150117)used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1 hour at room temperature.
