Anti-alpha smooth muscle Actin antibody [1A4] (FITC)

• Supplier: Abcam
• Catalog: ab8211
• Prices: $385.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: 1A4
• Applications: ICC/IF ICC/IF FC IHC-F IHC-P IHC-F WB
• Species Reactivities: Rat, Mouse, Sheep, Goat, Chicken, Guinea Pig, Cat, Dog, Human, Snake, Zebrafish
• Antigen: The N-terminal decapeptide of alpha-smooth muscle actin
• Description: Mouse Monoclonal
• Gene: ACTA2
• Conjugate: FITC

Product images

ICC/IF image of ab8211 stained SV40LT-SMC cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (FITC conjugated ab8211, 10µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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