All lanes : Anti-Amino-terminal enhancer of split antibody (ab16638) at 1 µg/mlLane 1 : As aboveLane 2 : As aboveLane 3 : As aboveLane 4 : As aboveLane 5 : As aboveLane 6 : Human Amino-terminal enhancer of split peptide (ab16684) at 1 µg/mlLane 7 : Human Amino-terminal enhancer of split peptide (ab16684) at 1 µg/mlLane 8 : Human Amino-terminal enhancer of split peptide (ab16684) at 1 µg/mlLane 9 : Human Amino-terminal enhancer of split peptide (ab16684) at 1 µg/mlLane 10 : Human Amino-terminal enhancer of split peptide (ab16684) at 1 µg/mlPerformed under reducing conditions.