Anti-AMPK alpha 2 antibody
| Name | Anti-AMPK alpha 2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3760 |
| Prices | $400.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP IHC-P |
| Species Reactivities | Mouse, Rat, Bovine, Human, Rabbit, Guinea Pig, Pig, Chimpanzee, Monkey, Ape, Hamster, Orangutan, Elephant |
| Antigen | Synthetic peptide (Human) - which represented a portion of AMP activated protein kinase, alpha-2 catalytic subunit encoded within exon 7 |
| Description | Rabbit Polyclonal |
| Gene | PRKAA2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab3760 staining AMPK alpha 2 in murine thymus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked with 2% BSA for 1 hour at 25°C followed by incubation with the primary antibody at a 1/100 dilution for 18 hours at 4°C. An HRP-conjugated anti-rabbit was used as secondary antibody at a 1/50 dilution. ab3760 presents in both the nuclear and the cytoplasm in mouse thymus.
ICC/IF image of ab3760 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3760, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-AMPK alpha 2 antibody (ab3760) at 1/1000 dilutionLane 1 : Murine hepatocytes cultured in Williams Medium E for 9 hoursLane 2 : Murine hepatocytes cultured in Williams Medium E for 57 hoursLane 3 : Murine hepatocytes cultured in Williams Medium E for 57 hours with 0.1% DMSOLysates/proteins at 24 µg per lane.SecondaryHRp conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilutiondeveloped using the ECL technique
Product References
Susceptibility to ATP depletion of primary proximal tubular cell cultures derived - Susceptibility to ATP depletion of primary proximal tubular cell cultures derived
Lieberthal W, Tang M, Zhang L, Viollet B, Patel V, Levine JS. BMC Nephrol. 2013 Nov 14;14:251.
AMPK protects proximal tubular cells from stress-induced apoptosis by an - AMPK protects proximal tubular cells from stress-induced apoptosis by an
Lieberthal W, Zhang L, Patel VA, Levine JS. Am J Physiol Renal Physiol. 2011 Dec;301(6):F1177-92. doi:
Skeletal muscle AMP-activated protein kinase is essential for the metabolic - Skeletal muscle AMP-activated protein kinase is essential for the metabolic
Lee-Young RS, Griffee SR, Lynes SE, Bracy DP, Ayala JE, McGuinness OP, Wasserman DH. J Biol Chem. 2009 Sep 4;284(36):23925-34.
Adenosine 5'-monophosphate-activated protein kinase promotes macrophage - Adenosine 5'-monophosphate-activated protein kinase promotes macrophage
Sag D, Carling D, Stout RD, Suttles J. J Immunol. 2008 Dec 15;181(12):8633-41.
AICAR inhibits adipocyte differentiation in 3T3L1 and restores metabolic - AICAR inhibits adipocyte differentiation in 3T3L1 and restores metabolic
Giri S, Rattan R, Haq E, Khan M, Yasmin R, Won JS, Key L, Singh AK, Singh I. Nutr Metab (Lond). 2006 Aug 10;3:31.