PRKAA2

Gene Symbol	PRKAA2
Entrez Gene	5563
Alt Symbol	AMPK, AMPK2, AMPKa2, PRKAA
Species	Human
Gene Type	protein-coding
Description	protein kinase, AMP-activated, alpha 2 catalytic subunit
Other Description	5'-AMP-activated protein kinase catalytic subunit alpha-2\|5'-AMP-activated protein kinase, catalytic alpha-2 chain\|ACACA kinase\|AMP-activated protein kinase alpha-2 subunit variant 2\|AMP-activated protein kinase alpha-2 subunit variant 3\|AMPK alpha 2\|AMPK subunit alpha-2\|AMPK-alpha-2 chain\|HMGCR kinase\|acetyl-CoA carboxylase kinase\|hydroxymethylglutaryl-CoA reductase kinase
Swissprots	Q9H1E8 Q9UD43 P54646
Accessions	AAB32732 EAX06650 P54646 AI732856 AK314011 BAG36722 AL596521 AL710110 BC043195 BC069680 AAH69680 BC069740 AAH69740 BC069823 AAH69823 BM979925 CB985850 CF552084 DA710012 EF056019 ABK30810 FJ200485 ACO54882 FJ200486 ACO54883 HQ628625 AER93375 U06454 AAA64745 NM_006252 NP_006243
Function	Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphoryl
Subcellular Location	Cytoplasm {ECO:0000250}. Nucleus {ECO:0000269\|PubMed:15866171}. Note=In response to stress, recruited by p53/TP53 to specific promoters.
Top Pathways	Glucagon signaling pathway, AMPK signaling pathway, Oxytocin signaling pathway, Non-alcoholic fatty liver disease (NAFLD), PI3K-Akt signaling pathway

AMPKα2 Antibody - 2757 from Cell Signaling Technology

Prices	$99.00, $246.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from HEK293 and COS cells, using AMPKα2 Antibody.

AMPKα2 (A-20) - sc-19129 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

AMPKα2 (A-20): sc-19129. Western blot analysis of AMPKα2 expression in mouse liver extract.

AMPKα2 (C-20) - sc-19131 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

AMPKα2 (C-20): sc-19131. Immunofluorescence staining of methanol-fixed L8 cells showing cytoplasmic localization.

p-AMPKα1 (Ser 496) - sc-101631 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

p-AMPKα1 (Ser 496): sc-101631. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing cytoplasmic localization.

Anti-AMPK alpha 2 (phospho S173) antibody - ab55886 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-AMPK alpha 2 (phospho S345) antibody [EPMDAR6] - ab129081 from Abcam

Prices	$313.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPMDAR6

All lanes : Anti-AMPK alpha 2 (phospho S345) antibody [EPMDAR6] (ab129081) at 1/1000 dilutionLane 1 : 293T cell lysate treated with Lambda PhosphataseLane 2 : 293T cell lysate, untreated Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-AMPK alpha 2 (phospho S491) antibody [EPR3052] - ab109402 from Abcam

Prices	$313.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR3052

All lanes : Anti-AMPK alpha 2 (phospho S491) antibody [EPR3052] (ab109402) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : 293T cell lysate treated with Lambda Phosphatase (LP)Lysates/proteins at 10 µg per lane.

Anti-AMPK alpha 2 antibody - ab3760 from Abcam

Prices	$400.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Anti-AMPK alpha 2 antibody - ab97275 from Abcam

Prices	$384.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

ab97275 at 1/200 dilution staining AMPK alpha 2 in paraformaldehyde-fixed A431 cells by immunofluorescence. The lower image shows ab97275 merged with a DNA probe.

Anti-AMPK alpha 2 antibody - ab105028 from Abcam

Prices	$376.00
Sizes	200 µl
Host	Goat
Clonality	Polyclonal

All lanes : Anti-AMPK alpha 2 antibody (ab105028) at 0.300000011921 µg/mlLane 1 : Mouse heart lysate in RIPA bufferLane 2 : Rat heart lysate in RIPA bufferLysates/proteins at 35 µg per lane.developed using the ECL technique

Anti-AMPK alpha 2 antibody - ab85953 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-AMPK alpha 2 antibody (ab85953) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 3 : A498 (Human Kidney Carcinoma) Whole Cell Lysate Lane 4 : Y79 (Human retinoblastoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-AMPK alpha 2 antibody - ab31959 from Abcam

Prices	$372.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-AMPK alpha 2 antibody - ab76792 from Abcam

Prices	$359.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-AMPK alpha 2 antibody (ab76792) at 1/50 dilutionLane 1 : 293 cell lysates, nontransfectedLane 2 : 293 cell lysates, transiently transfected with the AMPK alpha 2 gene Lysates/proteins at 2 µg per lane.

Anti-AMPK alpha 2 antibody - ab58171 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Western blot against tagged recombinant protein immunogen using ab58171 AMPK alpha 2 antibody at 1ug/ml.

Anti-AMPK alpha 2 antibody (Agarose) - ab19208 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PRKAA2 / AMPK Alpha 2 Antibody (aa353-367) - LS-C20490 from LifeSpan Bioscience

Prices	$475.00
Sizes	200 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PRKAA2 / AMPK Alpha 2 Antibody - LS-C20491 from LifeSpan Bioscience

Prices	$545.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PRKAA2 / AMPK Alpha 2 Antibody (C-Terminus) - LS-C20492 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRKAA2 / AMPK Alpha 2 Antibody (C-Terminus) - LS-C20493 from LifeSpan Bioscience

Prices	$385.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PRKAA2 / AMPK Alpha 2 Antibody (phospho-Ser175) - LS-C46806 from LifeSpan Bioscience

Prices	$275.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PRKAA2 / AMPK Alpha 2 Antibody - LS-C83009 from LifeSpan Bioscience

Prices	$435.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PRKAA2 / AMPK Alpha 2 Antibody (Agarose) - LS-C68918 from LifeSpan Bioscience

Prices	$635.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PRKAA2 / AMPK Alpha 2 Antibody (C-Terminus) - LS-C122646 from LifeSpan Bioscience

Prices	$465.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PRKAA2 / AMPK Alpha 2 Antibody - LS-C122651 from LifeSpan Bioscience

Prices	$385.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PRKAA2 / AMPK Alpha 2 Antibody - LS-C122652 from LifeSpan Bioscience

Prices	$355.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

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