Anti-AMPK beta 1 antibody [Y367]

Name	Anti-AMPK beta 1 antibody [Y367]
Supplier	Abcam
Catalog	ab32112
Prices	$391.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	Y367
Applications	WB IHC-P FC IP
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide corresponding to residues in Human AMPK beta 1
Description	Rabbit Monoclonal
Gene	PRKAB1
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-AMPK beta 1 antibody [Y367] (ab32112) at 1/5000 dilutionLane 1 : (A) : NIH 3T3.Lane 2 : (B) : Hela.Lane 3 : (C) : A431.Lane 4 : (D) : PC-12.

ab32112 at a 1:100 dilution staining AMPK beta 1 in human lung carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.

Overlay histogram showing HeLa cells stained with ab32112 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32112, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Product References

AMPK beta1 deletion reduces appetite, preventing obesity and hepatic insulin - AMPK beta1 deletion reduces appetite, preventing obesity and hepatic insulin

Dzamko N, van Denderen BJ, Hevener AL, Jorgensen SB, Honeyman J, Galic S, Chen ZP, Watt MJ, Campbell DJ, Steinberg GR, Kemp BE. J Biol Chem. 2010 Jan 1;285(1):115-22.

Association of AMP-activated protein kinase subunits with glycogen particles as - Association of AMP-activated protein kinase subunits with glycogen particles as

Bendayan M, Londono I, Kemp BE, Hardie GD, Ruderman N, Prentki M. J Histochem Cytochem. 2009 Oct;57(10):963-71.

Impaired skeletal muscle beta-adrenergic activation and lipolysis are associated - Impaired skeletal muscle beta-adrenergic activation and lipolysis are associated

Lessard SJ, Rivas DA, Chen ZP, van Denderen BJ, Watt MJ, Koch LG, Britton SL, Kemp BE, Hawley JA. Endocrinology. 2009 Nov;150(11):4883-91.

Hypothalamic CaMKK2 contributes to the regulation of energy balance. - Hypothalamic CaMKK2 contributes to the regulation of energy balance.

Anderson KA, Ribar TJ, Lin F, Noeldner PK, Green MF, Muehlbauer MJ, Witters LA, Kemp BE, Means AR. Cell Metab. 2008 May;7(5):377-88.