PRKAB1

Gene Symbol PRKAB1
Entrez Gene 5564
Alt Symbol AMPK, HAMPKb
Species Human
Gene Type protein-coding
Description protein kinase, AMP-activated, beta 1 non-catalytic subunit
Other Description 5'-AMP-activated protein kinase beta-1 subunit|5'-AMP-activated protein kinase subunit beta-1|AMP-activated protein kinase beta subunit|AMPK beta -1 chain|AMPK beta 1|AMPK subunit beta-1|AMPKb|protein kinase, AMP-activated, noncatalytic, beta-1
Swissprots Q9Y478 Q9UE20 Q9UEX2 Q9Y6V8 Q9UBV0
Accessions AAB71326 AAD00625 EAW98151 EAW98152 EAW98153 EAW98154 Q9Y478 AF022116 AAC98897 AJ224515 CAA12024 AK127820 AK301165 BAG62754 AK312495 BAG35397 BC001007 AAH01007 BC001056 AAH01056 BC001823 AAH01823 BC017671 AAH17671 BC018818 BU539177 BX537486 U83994 AAD09237 Y12556 CAA73146 XM_005253909 XP_005253966 NM_006253 NP_006244
Function Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Top Pathways Non-alcoholic fatty liver disease (NAFLD), Glucagon signaling pathway, AMPK signaling pathway, Oxytocin signaling pathway, Circadian rhythm

Phospho-AMPKβ1 (Ser182) Antibody - 4186 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from oligomycin treated C6 cells or C6 cell lysate treated with λ phosphatase, using Phospho-AMPKβ1 (Ser182) Antibody (upper) and AMPKβ1 Antibody #4182 (lower).

Phospho-AMPKβ1 (Ser108) Antibody - 4181 from Cell Signaling Technology

Prices $139.00, $279.00
Sizes 40 µl (4 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from C2C12 cells, untreated (lanes 1,3) or oligomycin-treated (lanes 2,4), using Phospho-AMPKβ1 (Ser108) Antibody (upper) or AMPKβ1 Antibody #4182 (lower). Cell lysates were treated with λ phosphatase in lanes 3 and 4 to demonstrate the phospho-specificity of Phospho-AMPKβ1 (Ser108) Antibody.

AMPKβ1 (71C10) Rabbit mAb - 4178 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 71C10
Western blot analysis of extracts from various cell types using AMPKβ1 (71C10) Rabbit mAb.

AMPKβ1/2 (57C12) Rabbit mAb - 4150 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 57C12
Western blot analysis of cell lysates from various cell lines using AMPKβ 1/2(57C12) Rabbit mAb.

AMPKβ1 Antibody - 12063 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 and COS-7 cells using AMPKβ1 Antibody.

AMPKβ1 Antibody - 4182 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

AMPKβ1 (G-20) - sc-19133 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AMPKβ1 (G-20): sc-19133. Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic and nuclear staining of glandular cells.

AMPKβ1 (H-75) - sc-20163 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

AMPKβ1 (N-18) - sc-19132 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AMPKβ1 (N-18): sc-19132. Western blot analysis of AMPKβ1 expression in NIH/3T3 (A), SW480 (B), C6 (C) and T98G (D) whole cell lysates.

AMPKβ1 (Z14) - sc-100357 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of AMPKβ1 phosphorylation in untreated (A,C) and lambda protein phosphatase treated (B,D) differentiated C2C12 whole cell lysates. Antibodies tested include p-AMPKβ1 (Ser 108): sc-33525 (A,C) and AMPKβ1 (Z14): sc-100357 (B,D).

p-AMPKβ1 (Ser 108) - sc-33525 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of AMPKβ1 phosphorylation in untreated (A,C) and lambda protein phosphatase treated (B,D) differentiated C2C12 whole cell lysates. Antibodies tested include p-AMPKβ1 (Ser 108): sc-33525 (A,C) and AMPKβ1 (Z14): sc-100357 (B,D).

p-AMPKβ1 (Ser 182) - sc-109906 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p-AMPKβ1 (Ser 182): sc-109906. Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic and nuclear staining of glandular cells.

Anti-AMPK beta 1 (phospho S108) antibody - ab156890 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AMPK beta 1 (phospho S108) antibody (ab156890) at 1/250 dilutionLane 1 : Human HEK293 Oligomycin 1µMLane 2 : Human HEK293 Oligomycin 1µM with Immunising peptide at 1/250 dilutionLane 3 : Human HEK293 Oligomycin 1µM with Control peptide at 1/250 dilutionLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-AMPK beta 1 (phospho S181) antibody - ab55311 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
This image shows human skeletal muscle tissue stained with ab55311 at 1/50 dilution. The left hand image shows untreated tissue, the right hand image shows tissue treated with the immunising phosphopeptide.

Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] - ab68206 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2181Y
All lanes : Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206) at 1/20000 dilutionLane 1 : human brain tissue lysate (cells were untreated)Lane 2 : human brain tissue lysate (cells were treated with AP)Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-AMPK beta 1 antibody - ab63533 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AMPK beta 1 antibody (ab63533) at 1/500 dilutionLane 1 : Extracts from COS7 cells (5-30ug), without immunising peptide.Lane 2 : Extracts from COS7 cells (5-30ug), with immunising peptide (5-10ug).

Anti-AMPK beta 1 antibody - ab79885 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AMPK beta 1 antibody (ab79885) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-AMPK beta 1 antibody - ab133213 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AMPK beta 1 antibody (ab133213) at 1/500 dilutionLane 1 : Extract from HepG2 cellsLane 2 : Extract from Jurkat cellsLane 3 : Extract from HuvEc cellsLane 4 : Extract from MCF-7 cellsLane 5 : Extract from HuvEc cells with immunizing peptide

Anti-AMPK beta 1 antibody - ab58175 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-AMPK beta 1 antibody (ab58175) + immunogen

Anti-AMPK beta 1 antibody [EP345] - ab108250 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP345
All lanes : Anti-AMPK beta 1 antibody [EP345] (ab108250) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : Fetal kidney lysateLane 3 : HeLa cell lysateLane 4 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 2000

Anti-AMPK beta 1 antibody [Y367] - ab32112 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y367
All lanes : Anti-AMPK beta 1 antibody [Y367] (ab32112) at 1/5000 dilutionLane 1 : (A) : NIH 3T3.Lane 2 : (B) : Hela.Lane 3 : (C) : A431.Lane 4 : (D) : PC-12.

Anti-AMPK beta 1 antibody - Aminoterminal end - ab70922 from Abcam

Prices $378.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AMPK beta 1 antibody - Aminoterminal end (ab70922) at 1/100 dilutionLane 1 : Jurkat cell lysateLane 2 : Mouse spleen tissue lysateLysates/proteins at 12.5 µg per lane.

Anti-PRKAB1 / AMPK Beta 1 Antibody (N-Terminus) - LS-C20495 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKAB1 / AMPK Beta 1 Antibody (phospho-Ser108) - LS-C20496 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKAB1 / AMPK Beta 1 Antibody (N-Terminus) - LS-C122647 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Search more