Anti-Angiopoietin-like 4 antibody [11F6C4]

• Supplier: Abcam
• Catalog: ab138526
• Prices: $376.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 11F6C4
• Applications: WB IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide derived from within residues 150 - 250 of Human Angiopoietin-like 4
• Description: Mouse Monoclonal
• Gene: ANGPTL4
• Conjugate: Unconjugated

Product images

IHC image of Angiopoietin-like 4 staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA based pH 9.0 solution(epitope retrieval solution 2) for 20 mins. The section was then incubated with ab138526, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

All lanes : Anti-Angiopoietin-like 4 antibody [11F6C4] (ab138526) at 5 µg/mlLane 1 : Adipose (Human) Total Protein Lysate - adult normal tissue (ab28980)Lane 2 : Skin (Human) Tissue Lysate - adult normal tissue (ab30166)Lane 3 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.