Anti-Angiotensin Converting Enzyme 1 antibody
| Name | Anti-Angiotensin Converting Enzyme 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab28311 |
| Prices | $388.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | The antibody is made to a synthetic peptide based on the first peptidase unit of human ACE1 (Human) |
| Description | Rabbit Polyclonal |
| Gene | ACE |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : Molecular weight markersLanes 2 - 4 : Anti-Angiotensin Converting Enzyme 1 antibody (ab28311) at 1/1000 dilutionLane 1 : As aboveLane 2 : ACE1 at 0.05 µgLane 3 : ACE1 at 0.01 µgLane 4 : ACE1 at 0.001 µgObserved band size : 150 kDa (why is the actual band size different from the predicted?)
ab28311 staining Angiotensin Converting Enzyme 1 antibody - Aminoterminal end catalytic domain in human mesenteric artery tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using TRIS pH 9.0, 123°C for 1 minute. Samples were then permeabilized using 0.1% Triton, blocked with 3% BSA for 30 minutes at 25°C and then incubated with ab28311 at a 1/500 dilution for 16 hours at 4°C. The secondary used was an undiluted goat anti-rabbit polyclonal-HRP.See Abreview