Anti-Annexin A2 antibody
| Name | Anti-Annexin A2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab54771 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-340 of Human Annexin II |
| Description | Mouse Monoclonal |
| Gene | ANXA2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Annexin II antibody (ab54771) used in immunofluorescence at 10ug/ml on HeLa cells.
Anti-Annexin A2 antibody (ab54771) at 1/1000 dilution + Whole cell lysate prepared from human Mesothelial cell line MeT-5A at 5 µgSecondaryHRP conjugated rabbit polyclonal to mouse IgG at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab54771 (2µg/ml) staining Annexin II in human prostate using an automated system (DAKO Autostainer Plus). Using this protocol there is strong membrane staining of glandular epithelium.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
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Maxwell GL, Hood BL, Day R, Chandran U, Kirchner D, Kolli VS, Bateman NW, Allard J, Miller C, Sun M, Flint MS, Zahn C, Oliver J, Banerjee S, Litzi T, Parwani A, Sandburg G, Rose S, Becich MJ, Berchuck A, Kohn E, Risinger JI, Conrads TP. Gynecol Oncol. 2011 Jun 1;121(3):586-94.
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Kratochwill K, Lechner M, Siehs C, Lederhuber HC, Rehulka P, Endemann M, Kasper DC, Herkner KR, Mayer B, Rizzi A, Aufricht C. J Proteome Res. 2009 Apr;8(4):1731-47.