Immunoprecipitation of p53 from A-431 whole cell lysate using p53 (C-19): sc-1311 (goat polyclonal antibody) followed by Western blot analysis using p53 (DO-1): sc-126 (mouse monoclonal antibody). Note presence of Protein A/G band using Protein A/G PLUS-Agarose: sc-2003 conventional IP matrix (A) as compared to their absence using ExactaCruz
TM B: sc-45039 (B).
p53 (C-19): sc-1311. Western blot analysis of p53 expression in non-transfected: sc-117752 (A) and mouse p53 transfected: sc-125766 (B) 293T whole cell lysates.
p53 (C-19): sc-1311. Western blot analysis of p53 expression in A-431 (A), WR19L (B) and mouse Lac Z (C) whole cell lysates.
p53 (C-19): sc-1311. Western blot analysis of p53 expression in non-transfected: sc-117752 (A) and mouse p53 transfected: sc-125766 (B) 293T whole cell lysates.
p53 (C-19): sc-1311. Western blot analysis of p53 expression in non-transfected: sc-117752 (A) and human p53 transfected: sc-158802 (B) 293T whole cell lysates.
p53 (C-19): sc-1311. Western blot analysis of p53 expression in A-431 whole cell lysate.
Western blot analysis of p53 expression in mouse Lac Z (A,B,C) whole cell lysate. Antibodies tested include p53 (C-19): sc-1311 (A), p53 (M-19): sc-1312 (B) and p53 (R-19): sc-1313 (C).