Western blot using ab3707 at 1/500.Lane 1: HeLa Nuclear ExtractLane 2: HeLa Whole Cell LysateLane 3: 293 Whole Cell LysateLane 4: A431 Whole Cell LysateLane 5: Jurkat Whole Cell LysateLane 6: HeLa Nuclear Extract + blocking/immunising peptideLane 7: HeLa Whole Cell Lysate + blocking/immunising peptideLane 8: 293 Whole Cell Lysate + blocking/immunising peptideLane 9: A431 Whole Cell Lysate + blocking/immunising peptideLane 10: Jurkat Whole Cell Lysate + blocking/immunising peptideAP2 alpha proteins (AP2A2 and AP2A1) have predicted molecular weights of 104 and 107kD. The band at just below 97kD (that is blocked by the immunising peptide) represents AP2 alpha, the lower bands are not blocked completely by the immunising peptide and are believed to be non-specific.Secondary ab: Rabbit polyclonal to Goat IgG HRPab6741 (1/5000)Exposure time: 1 minute.Lysates at 20µg/lane.Blocking peptide at 1µg/ml.