AP2A1

Gene Symbol AP2A1
Entrez Gene 160
Alt Symbol ADTAA, AP2-ALPHA, CLAPA1
Species Human
Gene Type protein-coding
Description adaptor-related protein complex 2, alpha 1 subunit
Other Description 100 kDa coated vesicle protein A|AP-2 complex subunit alpha-1|adapter-related protein complex 2 alpha-1 subunit|adapter-related protein complex 2 subunit alpha-1|adaptin, alpha A|adaptor protein complex AP-2 subunit alpha-1|adaptor-related protein complex 2 subunit alpha-1|alpha-adaptin A|alpha1-adaptin|clathrin assembly protein complex 2 alpha-A large chain|clathrin-associated/assembly/adaptor protein, large, alpha 1|plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
Swissprots Q96PP7 Q96PP6 O95782 Q9H070 Q96CI7
Accessions AAD15564 AAL11039 AAL11040 EAW52535 EAW52536 EAW52537 O95782 AK090661 AK094317 BAC04329 AK096757 AK124343 AK302005 BAG63408 AL136925 CAB66859 BC014214 AAH14214 DQ892253 ABM83179 DQ895454 ABM86380 XM_011526556 XP_011524858 XM_011526557 XP_011524859 NM_014203 NP_055018 NM_130787 NP_570603
Function Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to p
Subcellular Location Cell membrane {ECO:0000250}. Membrane, coated pit {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Note=AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV. {ECO:0000250}.
Tissue Specificity Isoform A expressed in forebrain, skeletal muscle, spinal cord, cerebellum, salivary gland, heart and colon. Isoform B is widely expressed in tissues and also in breast cancer and in prostate carcinoma cells.
Top Pathways Huntington's disease, Endocytosis, Endocrine and other factor-regulated calcium reabsorption, Synaptic vesicle cycle

α-Adaptin 1 (C-5) - sc-398024 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
α-Adaptin 1 (C-5): sc-398024. Western blot analysis of α-Adaptin 1 expression in NIH/3T3 (A), Hep G2 (B), KNRK (C) and c4 (D) whole cell lysates.

α-Adaptin 1 (E-15) - sc-249891 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

α-Adaptin 1 (P-16) - sc-249889 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

α-Adaptin 1/2 (AP.6) - sc-32284 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
α-Adaptin (AP.6): sc-32284. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing membrane localization.

α-Adaptin 1/2 (100/2) - sc-58215 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
α-Adaptin (100/2): sc-58215. Western blot analysis of α-Adaptin expression in Hep G2 (A), NIH/3T3 (B) and c4 (C) whole cell lysates.

Anti-alpha Adaptin antibody - ab186828 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-alpha Adaptin antibody (ab186828) at 1/500 dilutionLane 1 : 22RV1 cell extractsLane 2 : SK0V3 cell extractsLane 3 : Mouse brain cell extracts

Anti-alpha Adaptin antibody - ab91268 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-alpha Adaptin antibody (ab91268) at 1/100 dilution + HepG2 cell lysate at 35 µg

Anti-Alpha1-adaptin antibody [EPR7572] - ab151720 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7572
All lanes : Anti-Alpha1-adaptin antibody [EPR7572] (ab151720) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : HepG2 cell lysateLane 4 : A431 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-AP2 alpha antibody - ab156806 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-AP2 alpha antibody (ab156806) at 0.01 µg/ml + Human frontal cortex lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-AP2 alpha antibody - ab3707 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western blot using ab3707 at 1/500.Lane 1: HeLa Nuclear ExtractLane 2: HeLa Whole Cell LysateLane 3: 293 Whole Cell LysateLane 4: A431 Whole Cell LysateLane 5: Jurkat Whole Cell LysateLane 6: HeLa Nuclear Extract + blocking/immunising peptideLane 7: HeLa Whole Cell Lysate + blocking/immunising peptideLane 8: 293 Whole Cell Lysate + blocking/immunising peptideLane 9: A431 Whole Cell Lysate + blocking/immunising peptideLane 10: Jurkat Whole Cell Lysate + blocking/immunising peptideAP2 alpha proteins (AP2A2 and AP2A1) have predicted molecular weights of 104 and 107kD. The band at just below 97kD (that is blocked by the immunising peptide) represents AP2 alpha, the lower bands are not blocked completely by the immunising peptide and are believed to be non-specific.Secondary ab: Rabbit polyclonal to Goat IgG HRPab6741 (1/5000)Exposure time: 1 minute.Lysates at 20µg/lane.Blocking peptide at 1µg/ml.

Anti-AP2 alpha antibody - ab116289 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-AP2 alpha antibody (ab116289) at 1/100 dilution + 293 cell line lysates at 35 µg

Anti-AP2 alpha antibody [EPR7573(2)(B)] - ab170955 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7573(2)(B)
All lanes : Anti-AP2 alpha antibody [EPR7573(2)(B)] (ab170955) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : 293T cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Anti-AP2A1 / AP2-Alpha Antibody - LS-C125049 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-AP2A1 / AP2-Alpha Antibody - LS-C126612 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-AP2A1 / AP2-Alpha Antibody (clone AP6) - LS-C20296 from LifeSpan Bioscience

Prices $495.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone AP6

Anti-AP2A1 / AP2-Alpha Antibody (clone AC1-M11) - LS-C20294 from LifeSpan Bioscience

Prices $560.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone AC1-M11

Anti-AP2A1 / AP2-Alpha Antibody (clone Ada-1, Sepharose) - LS-C171305 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone Ada-1

Anti-AP2A1 / AP2-Alpha Antibody (clone Ada-1) - LS-C171303 from LifeSpan Bioscience

Prices $295.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone Ada-1

Anti-AP2A1 / AP2-Alpha Antibody (clone Ada-1, Magnetic beads) - LS-C171304 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone Ada-1

Anti-AP2A1 / AP2-Alpha Antibody (aa612-661) - LS-C135681 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
AP2A1 / AP2-Alpha antibody LS-C135681 Western blot of Fetal Brain lysate.

Anti-AP2A1 / AP2-Alpha Antibody (aa705-735, FITC) - LS-C251455 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AP2A1 / AP2-Alpha Antibody (aa705-735, AP) - LS-C251451 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AP2A1 / AP2-Alpha Antibody (aa705-735, Azide-free, HRP) - LS-C252865 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AP2A1 / AP2-Alpha Antibody (aa705-735, Biotin) - LS-C251452 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AP2A1 / AP2-Alpha Antibody (aa705-735, APC) - LS-C251453 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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