All lanes : Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138) at 1/20000 dilutionLane 1 : Human fetal brain LysateLane 2 : HepG2 cell LysateLane 3 : SH-SY5Y cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling AP3M2 with ab180138 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(Dylight 488) at 1/250 dilution. Conterstained with Dapi blue.
Flow cytometric analysis of 2% paraformaldehyde fixed U87-MG cells labeling AP3M2 with ab180138 at 1/30 dilution. Secondary antibody Goat anti rabbit IgG (FITC) at 1/75 dilution. Isotype control Rabbit IgG.
Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138) at 1/5000 dilution + U87-MG at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution