Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling AP3M2 with ab182000 at 1/50 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
All lanes : Anti-AP3M2 antibody [EPR12966] (ab182000) at 1/10000 dilutionLane 1 : HepG2 cell lysateLane 2 : U87-MG cell lysateLane 3 : SH-SY5Y cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L)-HRP at 1/1000 dilution
Flow cytometric analysis of SH-SY5Y cells (paraformaldehyde-fixed, 2%) labeling AP3M2 with ab182000 at 1/50 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.