Anti-APAF1 antibody
| Name | Anti-APAF1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2001 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide, corresponding to amino acids 12-28 of Human Apaf 1 |
| Description | Rabbit Polyclonal |
| Gene | APAF1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab2001 at 1µg/ml staining Apaf1 in human heart tissue by IHC
All lanes : Anti-APAF1 antibody (ab2001) at 1 µg/mlLane 1 : K562 cell lysate in absence of blocking peptideLane 2 : K562 cell lysate in presence of blocking peptide.Observed band size : 141 kDa (why is the actual band size different from the predicted?)
ICC/IF image of ab2001 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2001 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of Apaf1 in K562 cells using ab2001 at 10 ug/ml.
Product References
Identification of internal ribosome entry segment (IRES)-trans-acting factors for - Identification of internal ribosome entry segment (IRES)-trans-acting factors for
Cobbold LC, Spriggs KA, Haines SJ, Dobbyn HC, Hayes C, de Moor CH, Lilley KS, Bushell M, Willis AE. Mol Cell Biol. 2008 Jan;28(1):40-9. Epub 2007 Oct 29.
Subcellular localization of Apaf-1 in apoptotic rat pituitary cells. - Subcellular localization of Apaf-1 in apoptotic rat pituitary cells.
Potokar M, Kreft M, Chowdhury HH, Vardjan N, Zorec R. Am J Physiol Cell Physiol. 2006 Mar;290(3):C672-7. Epub 2005 Oct 5.
Sperm survival versus degradation in the Mammalian epididymis: a hypothesis. - Sperm survival versus degradation in the Mammalian epididymis: a hypothesis.
Jones R. Biol Reprod. 2004 Nov;71(5):1405-11. Epub 2004 Jun 23.