APAF1

Gene Symbol APAF1
Entrez Gene 317
Alt Symbol APAF-1, CED4
Species Human
Gene Type protein-coding
Description apoptotic peptidase activating factor 1
Other Description apoptotic protease-activating factor 1
Swissprots O43297 Q9UNC9 Q9UGN8 Q9UGN9 B2RMX8 Q9BXZ6 Q9UJ62 Q9UBZ5 Q7Z438 Q9UJ60 Q9UGP0 Q9UJ67 Q9UJ59 Q9UJ64 Q9UJ65 O14727 Q9UJ61 Q9UJ58 Q9UJ66 Q9UJ63
Accessions CAB65085 CAB65086 CAB65087 EAW97603 EAW97604 EAW97605 EAW97606 EAW97607 EAW97608 EAW97609 EAW97610 EAW97611 O14727 AB007873 BAA24843 AB103079 BAC77343 AF013263 AAC51678 AF134397 AAD38344 AF149794 AAD34016 AF248734 AAK28401 AJ243003 CAB55579 AJ243004 CAB55580 AJ243005 CAB55581 AJ243006 CAB55582 AJ243007 CAB55583 AJ243008 CAB55584 AJ243009 CAB55585 AJ243010 CAB55586 AJ243011 CAB55587 AJ243048 CAB55588 AJ243107 CAB56462 AK307509 BC136531 AAI36532 BC136532 AAI36533 BC150255 AAI50256 EF560718 ABQ59028 XM_011538252 XP_011536554 XM_011538253 XP_011536555 XM_011538254 XP_011536556 XM_011538255 XP_011536557 XR_429095 NM_001160 NP_001151 NM_013229 NP_037361 NM_181861 NP_863651 NM_181868 NP_863658 NM_181869 NP_863659
Function Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. {ECO:0000269|PubMed:10393175, ECO:0000269|PubMed:12804598}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:12804598}.
Tissue Specificity Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
Top Pathways Apoptosis, Huntington's disease, Amyotrophic lateral sclerosis (ALS), Tuberculosis, Alzheimer's disease

Apaf-1 (D7G4) Rabbit mAb - 8723 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D7G4
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® Apaf-1 siRNA I (+), using Apaf-1 (D7G4) Rabbit mAb #8723 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Apaf-1 (D7G4) Rabbit mAb confirms silencing of Apaf-1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

Apaf-1 (D5C3) Rabbit mAb - 8969 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5C3
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® Apaf-1 siRNA I #6229 (+), using Apaf-1 (D5C3) Rabbit mAb (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Apaf-1 (D5C3) Rabbit mAb confirms silencing of Apaf-1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

Apaf-1 (R205) Antibody - 5088 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from THP-1, ACHN, and 293 cells using Apaf-1 (R205) Antibody.

Apaf-1 Antibody - 4452 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Jurkat, PC12 and C2C12 cells, using Apaf-1 Antibody.

Apaf-1 (C-19) - sc-7231 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Apaf-1 (C-19): sc-7231. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue showing cytoplasmic staining of neuronal cells.

Apaf-1 (D-18) - sc-33870 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Apaf-1 (D-18): sc-33870. Western blot analysis of Apaf-1 expression in HUV-EC-C (A), ECV304 (B), K-562 (C), human PBL (D) and WI 38 (E) whole cell lysates.

Apaf-1 (H-324) - sc-8339 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Apaf-1 (H-324): sc-8339. Immunoperoxidase staining of formalin fixed, paraffin-embedded human upper stomach tissue showing cytoplasmic staining of glandular cells.

Apaf-1 (K-20) - sc-26685 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Apaf-1 (K-20): sc-26685. Western blot analysis of Apaf-1 expression in PC-12 (A), C2C12 (B) and human PBL (C) whole cell lysates.

Apaf-1 (N-19) - sc-7232 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Apaf-1 (T-17) - sc-33871 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Apaf-1 (13F11) - sc-135625 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal

Apaf-1 (18H2) - sc-135624 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal

Apaf-1 (2E12) - sc-135623 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal

Apaf-1 (2E10) - sc-65890 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Apaf-1 (2E10): sc-65890. Western blot analysis of expression in K-562 whole cell lysate.

Apaf-1 (5E11) - sc-65891 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Apaf-1 (5E11): sc-65891. Western blot analysis of Apaf-1 expression in Jurkat whole cell lysate.

Apaf-1 (24) - sc-135836 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Apaf-1 (24): sc-135836. Western blot analysis of Apaf-1 expression in human endothelial whole cell lysate.

Anti-APAF1 antibody - ab2001 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab2001 at 1µg/ml staining Apaf1 in human heart tissue by IHC 

Anti-APAF1 antibody - ab2000 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab2000 stained HEK293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2000, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-APAF1 antibody - ab2445 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-APAF1 antibody - ab53152 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with ab53152 at a dilution of 1/50-1/100. Right hand image: treated with immunising peptide. Left hand image: untreated.

Anti-APAF1 antibody - ab14228 from Abcam

Prices $376.00
Sizes 50 µg
Host Chicken
Clonality Polyclonal

Anti-APAF1 antibody - ab46899 from Abcam

Prices $376.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
Human testis stained with ab46899 using the antibody at a dilution of 1:200

Anti-APAF1 antibody [2E10] - ab36822 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2E10
Lane 1 : Anti-APAF1 antibody [2E10] (ab36822) at 0.25 µg/mlLane 2 : Anti-APAF1 antibody [2E10] (ab36822) at 0.5 µg/mlLane 1 : K562 cell lysateLane 2 : K562 cell lysate

Anti-APAF1 antibody [5E11] - ab36824 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5E11
ab36824 at 0.5µg/ml staining APAF1 in K562 cells by IHC

Anti-APAF1 antibody [E38] - ab32372 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E38
Anti-APAF1 antibody [E38] (ab32372) at 1/1000 dilution (purified) + Mouse spleen tissue lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
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